Abstract：
Age has been found to be one of the main risk factors for the severity and outcome of COVID-19. However, differences in SARS-CoV-2 specific antibody responses among COVID-19 patients of different age groups remain largely unknown. In this study, we analyzed the IgG/IgM responses to 21 SARS-CoV-2 proteins and 197 peptides that fully cover the spike protein against 731 sera collected from 731 COVID-19 patients aged from 1 to We show that there is no overall difference in SARS-CoV-2 antibody responses in COVID-19 patients in the 4 age groups. By antibody response landscape maps, we find that the IgG response profiles of SARS-CoV-2 proteins are positively correlated with age. The S protein linear epitope map shows that the immunogenicity of the S-protein peptides is related to peptide sequence, disease severity and age of the COVID-19 patients. Furthermore, the enrichment analysis indicates that low S1 IgG responses are enriched in patients aged <50 and high S1 IgG responses are enriched in mild COVID-19 patients aged >60. In addition, high responses of non-structural/accessory proteins are enriched in severe COVID-19 patients aged >70. These results suggest the distinct immune response of IgG/IgM to each SARS-CoV-2 protein in patients of different age, which may facilitate a deeper understanding of the immune responses in COVID-19 patients.
摘要：
年龄已被发现是COVID-19严重程度和结局的主要危险因素之一。然而,不同年龄组的COVID-19患者之间的SARS-CoV-2特异性抗体应答的差异仍然很大程度上未知.在这项研究中,我们分析了对21种SARS-CoV-2蛋白和197种肽的IgG/IgM应答,这些肽完全覆盖了731例1~1岁COVID-19患者的731份血清中的刺突蛋白。我们发现4个年龄组COVID-19患者的SARS-CoV-2抗体应答没有总体差异.根据抗体反应景观图，我们发现SARS-CoV-2蛋白的IgG应答谱与年龄呈正相关。S蛋白线性表位图显示S蛋白肽的免疫原性与肽序列有关,COVID-19患者的疾病严重程度和年龄。此外，富集分析表明，低S1IgG应答在<50岁的患者中富集，高S1IgG应答在>60岁的轻度COVID-19患者中富集。此外,非结构/辅助蛋白的高反应在年龄>70岁的严重COVID-19患者中富集。这些结果表明，在不同年龄的患者中，IgG/IgM对每种SARS-CoV-2蛋白的免疫反应不同。这可能有助于更深入地了解COVID-19患者的免疫反应。
