PDF(Pubmed)
Abstract：
Trichinellosis is a nematode-causing disease distinguished by its continuous transmission in the carnivores and omnivores. Despite effective eradication of the enteral forms, conventional drugs fail to eliminate the migrating and muscle ones. Over the past years, researchers intensified the work on herbal medicines as alternatives or aids to albendazole, the reference drug. This research hypothesizes that the therapeutic agent absorption route could be an evidence-based carrier molecule or auxiliary drug to albendazole. Accordingly, this in vitro study was designed to investigate mainly the phenotypic changes induced by a mono-treatment of albendazole, Lipidium sativum (garden cress), and Commiphora molmol (myrrh). Incredibly, no data were reported on the morphological alterations of T. spiralis larvae treated by any of these drugs. The experimental design tested various concentrations (25, 50, 100, and 200 μg/ml) of each herbal medicine for the lethal effects on the parasite forms for a day (1, 12, and 24h). The data showed that the highest significant mortality rate of the parasite forms was in favor of the concentration 200 μg/ml of both plant extracts in a time-dependent manner. Therefore, albendazole at 200 μg/ml dose was tested in parallel, and all experimental groups were compared to non-treated muscle larvae and worms. Albendazole-treated worms accounted for the least significant (p<0.001) survival rate (2 %), followed by myrrh (5 %), and the adverse was valid for the survival rate of the muscle larvae at that time. None of the larvae/worms was alive after 24 hours of incubation with the 200μg/ml of either treatment. The scanning electron microscope investigation of the experimental groups provided a shred of evidence for different routes of taking up the candidate drugs by the parasite. In conclusion, the results of the previous work in vivo and current in vitro study recommend myrrh over garden cress as a complementary agent of albendazole.
摘要：
旋毛虫病是一种引起线虫的疾病，其特征是在食肉动物和杂食动物中连续传播。尽管有效根除了肠内形式，常规药物不能消除迁移和肌肉的。在过去的几年里,研究人员加强了对草药作为阿苯达唑替代品或助剂的研究，参考药物。本研究假设治疗剂吸收途径可以是阿苯达唑的循证载体分子或辅助药物。因此，这项体外研究旨在研究阿苯达唑单治疗引起的表型变化，Lipidiumsativum(cardencress),和Commiphoramol（没药）。令人难以置信的是,没有关于这些药物治疗的旋毛虫幼虫形态改变的数据。实验设计测试了各种浓度（25、50、100和200μg/ml）的每种草药对一天（1、12和24h）的寄生虫形式的致死作用。数据显示,寄生虫形式的最高显著死亡率以时间依赖性方式有利于两种植物提取物的浓度为200μg/ml。因此,平行测试200μg/ml剂量的阿苯达唑，并将所有实验组与未治疗的肌肉幼虫和蠕虫进行比较。阿苯达唑处理的蠕虫占最低显着（p<0.001）存活率（2％），其次是没药(5%)，不利对当时肌肉幼虫的存活率有效。在用200μg/ml的任一处理孵育24小时后,幼虫/蠕虫都没有存活。对实验组的扫描电子显微镜调查为寄生虫摄取候选药物的不同途径提供了证据。总之,先前的体内研究和目前的体外研究结果推荐没药胜过豆瓣菜作为阿苯达唑的补充剂。
