PDF(Pubmed)
Abstract：
The human gut microbiota has been proposed to serve as a multifunctional organ in host metabolism, contributing effects to nutrient acquisition, immune response, and digestive health. Fasting during Ramadan may alter the composition of gut microbiota through changes in dietary behavior, which ultimately affects the contents of various metabolites in the gut. Here, we used liquid chromatography-mass spectrometry-based metabolomics to investigate the composition of fecal metabolites in Chinese and Pakistani individuals before and after Ramadan fasting. Principal component analysis showed distinct separation of metabolite profiles among ethnic groups as well as between pre- and post-fasting samples. After Ramadan fasting, the Chinese and Pakistani groups showed significant differences in their respective contents of various fecal metabolites. In particular, L-histidine, lycofawcine, and cordycepin concentrations were higher after Ramadan fasting in the Chinese group, while brucine was enriched in the Pakistani group. The KEGG analysis suggested that metabolites related to purine metabolism, 2-oxocarboxylic acid metabolism, and lysine degradation were significantly enriched in the total subject population pre-fasting vs. post-fasting comparisons. Several bacterial taxa were significantly correlated with specific metabolites unique to each ethnic group, suggesting that changes in fecal metabolite profiles related to Ramadan fasting may be influenced by associated shifts in gut microbiota. The fasting-related differences in fecal metabolite profile, together with these group-specific correlations between taxa and metabolites, support our previous findings that ethnic differences in dietary composition also drive variation in gut microbial composition and diversity. This landscape view of interconnected dietary behaviors, microbiota, and metabolites contributes to the future development of personalized, diet-based therapeutic strategies for gut-related disorders.
摘要：
人类肠道菌群已被提议作为宿主代谢中的多功能器官，对营养获取有影响，免疫反应，和消化健康。斋月期间的禁食可能会通过饮食行为的变化来改变肠道微生物群的组成，最终影响肠道中各种代谢物的含量。这里,我们使用基于液相色谱-质谱的代谢组学研究了中国人和巴基斯坦人在斋月禁食前后的粪便代谢物组成。主成分分析显示，种族之间以及禁食前后样本之间的代谢物分布有明显的分离。斋月后，中国和巴基斯坦组的各种粪便代谢物含量存在显着差异。特别是,L-组氨酸,lycofawcine,中国人群斋月后虫草素浓度较高，而巴基斯坦集团富含马钱子碱。KEGG分析表明与嘌呤代谢有关的代谢物，2-氧代羧酸代谢，和赖氨酸降解在禁食前的总受试者群体中显著富集禁食后比较。几个细菌类群与每个种族特有的特定代谢产物显着相关，这表明与斋月禁食相关的粪便代谢物谱的变化可能受到肠道微生物群相关变化的影响。粪便代谢物谱的空腹相关差异，以及分类群和代谢物之间的这些群体特异性相关性，支持我们先前的发现，即膳食组成的种族差异也会导致肠道微生物组成和多样性的变化。这种相互关联的饮食行为的景观视图，微生物群,和代谢物有助于个性化的未来发展，基于饮食的肠道相关疾病的治疗策略。
