Abstract:
Strain GY_HT was isolated from an artificial wetland in Okcheon, Chungcheongbuk-do Province, Republic of Korea. Strain GY_HT was closely related to Pseudolabrys taiwanensis CC-BB4T based on 16S rRNA gene sequences (94.7 % similarity) and clustered within the family Nitrobacteraceae. Cells of the isolate were Gram-stain-negative, catalase-negative and oxidase-positive, and colonies were white or pale transparent. A flagellum was observed, and the isolate could respire both aerobically and anaerobically. Growth of GY_ HT was observed in the following conditions: 10-45 °C, pH 5-11 and 0-4 % NaCl. The optimal conditions for growth were 25 °C, pH 6.5-7.5 and 0.5-1.5 % NaCl. The major fatty acids were C19 : 0 cyclo ω8c (35.8 %) and summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c; 27.4 %). The major quinone was found to be ubiquinone-10. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine were the major polar lipids. The G+C content of the genome of GY_HT was 63.3 mol%. Based on its phylogenomic, physiological and biochemical attributes, strain GY_HT represents a novel species of a novel genus of the family Nitrobacteraceae. We propose the name as Undibacter mobilis gen. nov., sp. nov. The type strain is GY_HT (=KCTC 62792T=JCM 32856T).
摘要:
从Okcheon的人工湿地中分离出菌株GY_HT,忠清北道,大韩民国。基于16SrRNA基因序列(相似性为94.7%),菌株GY_HT与假假山竹CC-BB4T密切相关,并聚集在硝化细菌科内。分离物的细胞为革兰氏染色阴性,过氧化氢酶阴性和氧化酶阳性,菌落呈白色或浅透明。观察到鞭毛,分离物可以有氧和厌氧呼吸。在以下条件下观察到GY_HT的生长:10-45°C,pH5-11和0-4%NaCl。生长的最佳条件是25°C,pH6.5-7.5和0.5-1.5%NaCl。主要脂肪酸为C19:0环状ω8c(35.8%),合计特征为8(C18:1ω7c/C18:1ω6c;27.4%)。发现主要的醌是泛醌-10。二磷脂酰甘油,磷脂酰乙醇胺,磷脂酰甘油和磷脂酰胆碱是主要的极性脂质。GY_HT基因组的G+C含量为63.3mol%。基于它的系统发育,生理和生化属性,菌株GY_HT代表硝基杆菌科的新属的新物种。我们建议名称为Undibactermobilisgen。11月。,sp.11月。菌株类型为GY_HT(=KCTC62792T=JCM32856T)。