Abstract：
Herein, a facile and sensitive dual mode sensing strategy for amantadine (AMD) level evaluation by coupling the plasmonic Au nanorod (NR) and supramolecular SH-cyclodextrin (CD) through strong Au-S bond is proposed. Methylene blue (MB) molecules can be inserted into the cavity of CD molecules through the hydrophobic interaction, which would cause the plasmon resonance energy transfer (PRET) process as well as electrochemical signal response due to the spectrum overlap between Au NR and MB molecules and the electrochemical conversion activity of MB molecules. Subsequently, AMD would induce the replacement of MB molecules because of the stronger interaction with CD, resulting the recovery of scattering intensity of Au NR and decrease of the electrooxidation current of MB. On one hand, the increase of Au NR scattering intensity is linearly proportional to AMD with the concentration from 0.4 to 3.0 μM, and reaches a limit of detection (LOD) of 0.28 μM. On the other hand, electrochemical measurement method enlarged the detection range of AMD. The variation of electrochemical oxidation peak current of MB is linearly proportional to the logarithm value of AMD concentration from 2.5 to 375.0 μM, with LOD of 1.9 μM. Subsequently, the proposed dual mode response sensing strategy was successfully employed for the detection of AMD in human serum samples with great selectivity and sensitivity, with a recovery percentage ranged from 92.6 to 112.0%. Overall, this Au NR@SH-CD-MB nanoparticle based single particle plasmonic and electrochemical dual mode sensing method provides great potential in the field of clinical drug detection or metabolic process investigation in the future.
摘要：
在这里，提出了一种通过强Au-S键耦合等离子体Au纳米棒（NR）和超分子SH-环糊精（CD）来评估金刚烷胺（AMD）水平的简便而灵敏的双模式传感策略。亚甲基蓝(MB)分子可以通过疏水作用插入到CD分子的空腔中,由于AuNR和MB分子之间的光谱重叠以及MB分子的电化学转换活性，这将导致等离子体共振能量转移（PRET）过程以及电化学信号响应。随后，由于与CD的相互作用更强，AMD会诱导MB分子的替换，导致AuNR的散射强度恢复和MB的电氧化电流降低。一方面,AuNR散射强度的增加与AMD成线性比例，浓度从0.4到3.0μM，并且达到0.28μM的检测限(LOD)。另一方面,电化学测量方法扩大了AMD的检测范围。MB的电化学氧化峰电流的变化与AMD浓度从2.5到375.0μM的对数值成线性比例，LOD为1.9μM。随后，提出的双模式响应传感策略成功地用于检测人血清样品中的AMD，具有很高的选择性和灵敏度，回收率为92.6％至112.0％。总的来说,这种基于AuNR@SH-CD-MB纳米粒子的单粒子等离子体和电化学双模式传感方法在未来的临床药物检测或代谢过程研究领域提供了巨大的潜力。
