Abstract：
BACKGROUND: A profound understanding of the evolution and anatomy of the viscero- and neurocranium is quintessentially important for orthodontists. This particularly alludes to structures, which are directly targeted by orthodontic therapy such as the maxilla and the mid-palatal suture. The anatomy of the mid-palatal suture of toothed individuals is well described, whereas little is known about sutures\' morphological changes after tooth loss. Therefore, the aim of this study was to evaluate the edentulous mid-palatal suture by means of histologic and histomorphometric analysis.
METHODS: Ten mid-palatal sutures of edentulous donors as well as six age- and sex matched dentulous controls were examined. For the histological and histomorphometric analysis (sutural width, obliteration, vascularization and interdigitation) conventional staining protocols (HE, Movat-Pentachrome, Sirius Red) and immunofluorescence (vWF, TRAP) were performed. Histomorphometric analysis was carried out using NIS-elements imaging software.
RESULTS: When compared to dentulous controls, the edentulous investigation group showed a decreased vascularization and sutural width as well as an increased sutural obliteration. Notably, a high variability and inhomogeneity within regard the histomorphometric parameters was seen in edentulous samples.
CONCLUSIONS: The mid-palatal suture of edentulous individuals showed significant morphological differences compared to individuals with toothed jaws. The loss of teeth and thereby functional loading seems to have a considerable impact on sutures\' morphology.
METHODS: Ten mid-palatal sutures of edentulous donors as well as six age- and sex matched dentulous controls were examined. For the histological and histomorphometric analysis (sutural width, obliteration, vascularization and interdigitation) conventional staining protocols (HE, Movat-Pentachrome, Sirius Red) and immunofluorescence (vWF, TRAP) were performed. Histomorphometric analysis was carried out using NIS-elements imaging software.
RESULTS: When compared to dentulous controls, the edentulous investigation group showed a decreased vascularization and sutural width as well as an increased sutural obliteration. Notably, a high variability and inhomogeneity within regard the histomorphometric parameters was seen in edentulous samples.
CONCLUSIONS: The mid-palatal suture of edentulous individuals showed significant morphological differences compared to individuals with toothed jaws. The loss of teeth and thereby functional loading seems to have a considerable impact on sutures\' morphology.
摘要：
背景：对内脏和神经颅骨的进化和解剖结构的深刻理解对于正畸医生至关重要。这特别暗示了结构，正畸治疗直接靶向，如上颌骨和中腭缝合。很好地描述了带齿个体的中腭缝合线的解剖结构，而对牙齿脱落后缝合线的形态变化知之甚少。因此,这项研究的目的是通过组织学和组织形态学分析来评估无牙颌中腭缝合。
方法：检查了十条无牙供体的腭中缝合线以及六个年龄和性别匹配的无牙对照。对于组织学和组织形态学分析(缝合宽度，抹杀，血管化和交叉)常规染色方案(HE，Movat-Pentachrome,天狼星红)和免疫荧光(vWF，进行TRAP）。使用NIS-元件成像软件进行组织形态计量学分析。
结果：与牙体对照相比，无牙调查组显示血管形成和缝合宽度减少，缝合闭塞增加。值得注意的是,在无牙样本中观察到组织形态参数的高度变异性和不均匀性。
结论：无牙颌个体的中腭缝合与有牙颌的个体相比显示出显著的形态学差异。牙齿的丧失和功能负荷似乎对缝合线的形态有相当大的影响。
方法：检查了十条无牙供体的腭中缝合线以及六个年龄和性别匹配的无牙对照。对于组织学和组织形态学分析(缝合宽度，抹杀，血管化和交叉)常规染色方案(HE，Movat-Pentachrome,天狼星红)和免疫荧光(vWF，进行TRAP）。使用NIS-元件成像软件进行组织形态计量学分析。
结果：与牙体对照相比，无牙调查组显示血管形成和缝合宽度减少，缝合闭塞增加。值得注意的是,在无牙样本中观察到组织形态参数的高度变异性和不均匀性。
结论：无牙颌个体的中腭缝合与有牙颌的个体相比显示出显著的形态学差异。牙齿的丧失和功能负荷似乎对缝合线的形态有相当大的影响。
