PDF(Pubmed)
Abstract：
Skin wound healing is an important clinical challenge, and the main treatment points are accelerating epidermal regeneration and preventing infection. Therefore, it is necessary to develop a wound dressing that can simultaneously cure bacterial infections and accelerate wound healing. Here, we report a multifunctional composite wound dressing loaded with chitosan (CS)-binding bFGF (CSBD-bFGF) and antimicrobial peptides (P5S9K). First, CS was used as the dressing matrix material, and P5S9K was encapsulated in CS. Then, CSBD-bFGF was designed by combining recombinant DNA technology and tyrosinase treatment and modified on the dressing material surface. The results show that the binding ability of CSBD-bFGF and CS was significantly improved compared with that of commercial bFGF, and CSBD-bFGF could be controllably released from the CS dressing. More importantly, the prepared dressing material showed excellent antibacterial activity in vivo and in vitro and could effectively inhibit the growth of E. coli and S. aureus. Using NIH3T3 cells as cellular models, the results showed that the CSBD-bFGF@CS/P5S9K composite dressing was a friendly material for cell growth. After cells were seeded on the composite dressing surface, collagen-1 (COL-1) and vascular endothelial growth factor (VEGF) genes expression in cells were significantly upregulated. Finally, the full-thickness wound of the rat dorsal model was applied to analyse the tissue repair ability of the composite dressing. The results showed that the composite dressing containing CSBD-bFGF and P5S9K had the strongest ability to repair skin wounds. Therefore, the CSBD-bFGF@CS/P5S9K composite dressing has good antibacterial and accelerated wound healing abilities and has good application prospects in the treatment of skin wounds.
摘要：
皮肤伤口愈合是一个重要的临床挑战，主要治疗要点是加速表皮再生和预防感染。因此,有必要开发一种可以同时治愈细菌感染并加速伤口愈合的伤口敷料。这里,我们报道了一种负载有壳聚糖（CS）结合bFGF（CSBD-bFGF）和抗菌肽（P5S9K）的多功能复合伤口敷料。首先,CS被用作敷料基质材料，P5S9K封装在CS中。然后，CSBD-bFGF是通过结合重组DNA技术和酪氨酸酶处理设计的，并在敷料材料表面进行修饰。结果表明，与市售bFGF相比，CSBD-bFGF与CS的结合能力明显提高，CSBD-bFGF可以从CS敷料中可控地释放。更重要的是,所制备的敷料材料具有良好的体内外抗菌活性,能有效抑制大肠杆菌和金黄色葡萄球菌的生长。使用NIH3T3细胞作为细胞模型，结果表明，CSBD-bFGF@CS/P5S9K复合敷料是细胞生长的友好材料。将细胞接种在复合敷料表面后，胶原-1（COL-1）和血管内皮生长因子（VEGF）基因在细胞中的表达显著上调。最后,应用大鼠背侧全层创面模型分析复合敷料的组织修复能力。结果表明，含CSBD-bFGF和P5S9K的复合敷料对皮肤创面的修复能力最强。因此,CSBD-bFGF@CS/P5S9K复合敷料具有良好的抗菌和加速伤口愈合能力，在皮肤伤口治疗中具有良好的应用前景。
