PDF(Pubmed)
Abstract：
Therapeutic ultrasound was administered to patients suffering from bone fracture with FDA approval. Bone and cartilage are piezoelectric materials. To investigate the effects of piezoelectricity on the cells of chondrogenic lineage, we applied ultrasound stimulation on an AT-cut quartz coverslip to generate electric field fluctuations. The bone-marrow-derived mesenchymal stem cells (BMMSC) and primary chondrocytes were cultured on either glass or quartz coverslips for ultrasound stimulation. The cells were immunofluorescent-labeled for the assessment of cell arrangement and ciliary orientation. Ultrasound and piezoelectricity both stimulate cell migration and disrupt ciliary orientation induced by directional migration. In particular, piezoelectric effects on cell rearrangement can be abolished by the inhibitor specifically targeting atypical Protein kinase C zeta (PKCζ). Our findings shed light on the possibility of cellular modulation by using piezoelectric manipulation.
摘要：
经FDA批准,对患有骨折的患者施用治疗性超声。骨和软骨是压电材料。为了研究压电对软骨谱系细胞的影响，我们在AT切割石英盖玻片上施加超声刺激以产生电场波动。将骨髓来源的间充质干细胞（BMMSC）和原代软骨细胞培养在玻璃或石英盖玻片上进行超声刺激。对细胞进行免疫荧光标记以评估细胞排列和纤毛取向。超声和压电都刺激细胞迁移并破坏由定向迁移引起的纤毛取向。特别是,特异性靶向非典型蛋白激酶Cζ（PKCζ）的抑制剂可以消除对细胞重排的压电效应。我们的发现揭示了通过使用压电操纵进行细胞调制的可能性。
