PDF(Pubmed)
Abstract：
Indigo naturalis, a herbal medicine purified from indigo-containing plants, such as Strobilanthes cusia, Isatis tinctoria, and Polygonum tinctorium, has been reported to be useful in the treatment of ulcerative colitis by activating the aryl hydrocarbon receptor. However, the aryl hydrocarbon receptor pathway causes crucial side effects, such as pulmonary arterial hypertension. Although P. tinctorium is one of the plant derivatives of indigo naturalis, it is not identical to it. To date, the pure leaves of P. tinctorium have not been reported to ameliorate ulcerative colitis. Therefore, we investigated the effect of pure P. tinctorium leaves, which are consumed in some regions, on experimental colitis induced in mice using sodium dextran sulfate. We found that P. tinctorium leaves ameliorated weight loss (P < 0.01) and pathological inflammatory changes in the colon (P < 0.05), enhanced mRNA expression of interleukin-10 (P < 0.05), and decreased expression of tumor necrosis factor-in colonic tissues (P < 0.05), as determined using quantitative real-time reverse transcription polymerase chain reaction. The intraperitoneal administration of an aryl hydrocarbon receptor antagonist did not antagonize the inhibition of mucosal destruction, whereas an anti-interleukin-10 receptor antibody did. These results suggest that P. tinctorium ameliorate sodium dextran sulfate-induced intestinal inflammation via interleukin-10-related pathway, independent of the aryl hydrocarbon receptor pathway. P. tinctorium leaves have the potential to be a new, safe treatment for ulcerative colitis.
摘要：
靛蓝，从含有靛蓝的植物中纯化的草药，例如Strobilanthescusia，板蓝花tinctoria,和Polygonumtinctorium，据报道,通过激活芳基烃受体可用于治疗溃疡性结肠炎。然而,芳香烃受体途径引起关键的副作用，如肺动脉高压。虽然P.tinctorium是靛蓝的植物衍生物之一，它与它不相同。迄今为止,纯叶的P.tinctorium尚未报道改善溃疡性结肠炎。因此,我们调查了纯丁香叶的影响，在一些地区消费，用葡聚糖硫酸钠诱导小鼠实验性结肠炎。我们发现P.tintorium叶改善了体重减轻（P<0.01）和结肠病理性炎症改变（P<0.05）。白细胞介素-10mRNA表达增强(P<0.05),结肠组织中肿瘤坏死因子的表达降低(P<0.05),使用定量实时逆转录聚合酶链反应确定。芳基烃受体拮抗剂的腹膜内给药没有拮抗粘膜破坏的抑制作用，而抗白细胞介素-10受体抗体确实如此。这些结果表明,P.tinctorium通过白细胞介素-10相关途径改善葡聚糖硫酸钠诱导的肠道炎症,独立于芳烃受体途径。P.tinctorium叶子有可能成为一种新的，溃疡性结肠炎的安全治疗。
