PDF(Pubmed)
Abstract：
Positron emission tomography (PET) can accurately locate and quantify radioactivity over traditional single photon emission computed tomography (SPECT), encouraging its application in kidney function evaluation and glomerular filtration rate (GFR) measurement. 68Ga-ethylenediamine-tetraacetic acid (68Ga-EDTA) is a novel PET tracer for renal scan but a mature GFR calculation method still pending establishment. Herein, we aim to investigate the imaging performance of 68Ga-EDTA dynamic PET in healthy C57BL/6 mice, establish quantitative methods to calculate GFR, and evaluate its feasibility in mice with kidney dysfunction. Dynamic PET of 68Ga-EDTA successfully visualized the whole process of tracer elimination. GFR values were measured by the integral method (253.80±40.11 μL/min) and the Patlak Plot method (22.69±9.75 μL/min), while blood clearance rate of the tracer was found at 787.46±70.86 μL/min. The PET-based GFR values correlate well with the GFRblood (R2=0.7468, R2=0.8793). The Integral method provides better accuracy than Patlak Plot method. Further application of GFR measurement in kidney-diseased mice proves better performance of the Integral method for defining split renal function.
摘要：
正电子发射断层扫描(PET)相对于传统的单光子发射计算机断层扫描(SPECT)可以准确地定位和量化放射性,鼓励其在肾功能评估和肾小球滤过率（GFR）测量中的应用。68Ga-乙二胺四乙酸（68Ga-EDTA）是一种用于肾脏扫描的新型PET示踪剂，但成熟的GFR计算方法仍有待建立。在这里，我们旨在研究68Ga-EDTA动态PET在健康C57BL/6小鼠中的成像性能，建立定量方法来计算GFR，并评估其在肾功能不全小鼠中的可行性。68Ga-EDTA的动态PET成功地可视化了示踪剂消除的整个过程。GFR值通过积分法(253.80±40.11μL/min)和PatlakPlot法(22.69±9.75μL/min)测量,示踪剂的血液清除率为787.46±70.86μL/min。基于PET的GFR值与GFR血良好相关(R2=0.7468,R2=0.8793)。积分方法比PatlakPlot方法具有更好的精度。GFR测量在肾脏疾病小鼠中的进一步应用证明了用于定义分裂肾功能的积分方法的更好性能。
