PDF(Pubmed)
Abstract：
Cervical cancer is the second most lethal cancer in Indonesia, behind breast cancer. One of the reasons cancer cells are difficult to treat is that the immune system is sometimes unable to recognise them as foreign. Cytokinin therapy is carried out so that the immune system can strengthen its response to cancer cells, with the aim of slowing or stopping the development of malignant cells. Zanthoxylum acanthopodium DC, also known as andaliman, is an Indonesian herb and a member of the Rutaceae family. It is rich in antioxidants and has anti-inflammatory and anti-cancer properties. The current study aimed to investigate the histological changes and changes in the expression of cytokines, such as IL-10, IL1β, VEGFR1, and TGFβ1, associated with andaliman treatment. Sample tissues and serums extracted from cervical cancer rat models were used. Rats were divided into five groups: a control group (C-), cancer model group (C+), cancer with a dose of Z. acanthopodium methanolic extract (ZAM) 100 mg/body weight (BW) ZAM (ZAM100), cancer with a dose of ZAM 200 mg/BW ZAM (ZAM200), and cancer with a dose of ZAM 400 mg/BW ZAM (ZAM400). Treatment lasted for 1 month. Blood samples were prepared for ELISA analysis, and cervical tissue was stained for immunohistochemistry using antibodies against IL-10, IL-1β, VEGFR1, and TGFβ1. Administration of ZAM had no significant effect on rat body weight and cervical organs (p > 0.05). However, it impacted haematological parameters in rats with cervical cancer (p < 0.05). Elevated malondialdehyde levels may be linked to superoxide dismutase deficiency in tumour tissue. ZAM significantly decreased the expression of IL1β, TGFβ1, and VEGFR1 (p < 0.01), while it increased the expression of IL-10. Therefore, ZAM may be a potential target for molecular cytokine therapy for cervical cancer.
摘要：
宫颈癌是印度尼西亚第二大致命癌症，乳腺癌背后癌细胞难以治疗的原因之一是免疫系统有时无法将它们识别为异物。细胞分裂素治疗是为了让免疫系统加强对癌细胞的反应，目的是减缓或阻止恶性细胞的发展。花椒DC，也被称为安达里曼，是印度尼西亚草本植物，是芦荟科的成员。它富含抗氧化剂，具有抗炎和抗癌特性。本研究旨在探讨组织学改变和细胞因子表达的变化,如IL-10，IL1β，VEGFR1和TGFβ1与安达利曼治疗相关。使用从宫颈癌大鼠模型中提取的样品组织和血清。将大鼠分为五组：对照组（C-），癌症模型组（C+），癌症与一个剂量的Z.刺五加甲醇提取物（ZAM）100毫克/体重（BW）ZAM（ZAM100），ZAM200mg/BWZAM（ZAM200）剂量的癌症，和ZAM400mg/BWZAM（ZAM400）剂量的癌症。治疗持续1个月。制备血样用于ELISA分析,宫颈组织使用抗IL-10，IL-1β的抗体进行免疫组织化学染色，VEGFR1和TGFβ1。ZAM给药对年夜鼠体重和宫颈器官无显著影响(p>0.05)。然而,它影响了宫颈癌大鼠的血液学参数（p<0.05）。丙二醛水平升高可能与肿瘤组织中的超氧化物歧化酶缺乏有关。ZAM显著降低IL1β的表达,TGFβ1和VEGFR1（p<0.01），同时增加了IL-10的表达。因此,ZAM可能是宫颈癌分子细胞因子治疗的潜在靶点。
