PDF(Pubmed)
Abstract：
The present study illustrates the transformation ability of two wild-type bacterial strains of Rhizobium rhizogenes (MTCC 532 and MTCC 2364) on the embryogenic callus and callus-derived plantlets of a threatened Indian orchid, Dendrobium ovatum. Co-culture of the bacterium with the explants gave marginal hairy root phenotype that failed to multiply in the culture medium. Some primary and secondary metabolites were subdued in infected explants. Moscatilin, the stilbenoid active principle in D. ovatum, was found below the detection limit. The presence of two metabolites viz., Laudanosine, a benzyltetrahydroisoquinoline alkaloid and Lyciumin B, a cyclic peptide, were detected exclusively in the infected explants. The subjugated amino acids and phenolics in the infected plantlets were routed to produce phytoanticipins, and phenanthrenes, strengthening the defence mechanism in infected tissues. This research implies that the plant\'s defence mechanism activation could have prevented the extensive hairy root formation in the explants, even though nodulations and phenotype transitions were witnessed. Moscatilin has a structural resemblance with Resveratrol, a phytoalexin that combats bacterial and fungal pathogens. The study favours the possibility of Moscatlin being a precursor for phenanthrene compounds, thereby serving as a \'phytoanticipin\' during the infection phase.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-022-03180-9.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-022-03180-9.
摘要：
本研究说明了根瘤菌的两种野生型菌株（MTCC532和MTCC2364）对受威胁的印度兰花的胚性愈伤组织和愈伤组织衍生的小植株的转化能力，卵石斛。细菌与外植体的共培养产生边缘毛状根表型，但在培养基中未能繁殖。在感染的外植体中，一些初级和次级代谢产物被抑制。莫斯卡蒂林,卵形D.中的二苯乙烯类活性成分，被发现低于检测限。两种代谢物的存在。,Laudanosine,苄基四氢异喹啉生物碱和LyciuminB，环状肽，仅在感染的外植体中检测到。被感染的小植株中的共轭氨基酸和酚类被引导产生植物预期蛋白，和菲，加强受感染组织的防御机制。这项研究表明，植物的防御机制激活可能阻止了外植体中广泛的毛状根形成，即使出现结节和表型转变。莫斯卡提林与白藜芦醇结构相似，一种对抗细菌和真菌病原体的植物抗毒素。该研究支持Moscatlin成为菲化合物前体的可能性，从而在感染阶段充当“植物预期素”。
UNASSIGNED：在线版本包含补充材料，可在10.1007/s13205-022-03180-9获得。
UNASSIGNED：在线版本包含补充材料，可在10.1007/s13205-022-03180-9获得。
