Abstract：
Pediatric acute respiratory distress syndrome (PARDS) is a severe form of respiratory failure associated with high mortality among children. The objective of this study is reported to explore the clinical function and molecular roles of microRNA-101-3p (miR-101-3p) in PARDS. The levels of miR-101-3p and mRNA levels of SRY-related high-mobility group box 9 (Sox9) were measured by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Additionally, the diagnostic role of miR-101-3p was identified by using the Receiver operating characteristic (ROC) curve. The cell proliferation and apoptosis were examined through Cell Counting Kit-8 (CCK-8) assay and flow cytometry. To detect inflammation in cells, enzyme-linked immunosorbent assays (ELISA) were performed. The target gene of miR-101-3p was confirmed through data obtained from the luciferase activity. In patients with PARDS, miR-101-3p expression was decreased. Moderate and severe PARDS patients had lower levels of miR-101-3p than mild PARDS patients. The inflammatory progression was related to the aberrant expression of miR-101-3p in all PARDS children. MiR-101-3p was highly predictive for the detection of children with PARDS. In addition, miR-101-3p might protect A549 cells from abnormal proliferation, apoptosis, and inflammation caused by lipopolysaccharide (LPS). Sox9 might be a target gene of miR-101-3p and increased mRNA expression of Sox9 in LPS-treated A549 cells was inhibited by overexpression of miR-101-3p. Ultimately, this study suggested that reduced expression of miR-101-3p plays a role in PARDS, providing a novel angle to study the disease.
摘要：
小儿急性呼吸窘迫综合征（PARDS）是一种与儿童高死亡率相关的严重呼吸衰竭。本研究旨在探讨microRNA-101-3p（miR-101-3p）在PARDS中的临床功能和分子作用。通过定量逆转录聚合酶链反应(RT-qPCR)检测miR-101-3p水平和SRY相关高迁移率族蛋白9(Sox9)mRNA水平。此外,miR-101-3p的诊断作用通过接收器工作特征(ROC)曲线进行鉴定.通过细胞计数试剂盒-8(CCK-8)测定和流式细胞术检测细胞增殖和凋亡。为了检测细胞中的炎症，进行酶联免疫吸附测定(ELISA)。通过从荧光素酶活性获得的数据确认miR-101-3p的靶基因。在PARDS患者中，miR-101-3p表达降低。中度和重度PARDS患者的miR-101-3p水平低于轻度PARDS患者。炎症进展与所有PARDS儿童中miR-101-3p的异常表达有关。MiR-101-3p对PARDS患儿的检测具有高度预测性。此外,miR-101-3p可能保护A549细胞免受异常增殖,凋亡，和脂多糖（LPS）引起的炎症。Sox9可能是miR-101-3p的靶基因，miR-101-3p的过表达抑制了LPS处理的A549细胞中Sox9的mRNA表达增加。最终,这项研究表明，miR-101-3p的表达降低在PARDS中起作用，为研究该病提供了一个新的角度。
