Abstract：
Aichivirus C is an emerging virus in goats, but its biological significance remains unknown. In this study, 18 diarrheic and 16 non-diarrheic faecal samples of kids were collected from a farm with an on-going diarrheic outbreak in Sichuan Province, China in May 2021. Of these samples, 77.8% (14/18) of diarrheic samples were detected as Aichivirus C positive by RT-PCR, which was significantly higher than that of non-diarrheic faces (0%, p < .001); meanwhile, other common diarrhoea-causing pathogens in goats were not detected in diarrheic samples, except for two samples that were detected as caprine enterovirus positive, suggesting that Aichivirus C was associated with goat diarrhoea. Furthermore, five Aichivirus C strains were successfully isolated from positive samples using Vero cell lines and two isolates were further plaque-purified, named SWUN/F5/2021(10-6.7 TCID50 /0.1 mL) and SWUN/F6/2021(10-7 TCID50 /0.1 mL). Interestingly, Aichivirus C strain could cause systemic infection in experimental kids via oral administration, with the main clinical manifestation being severe watery diarrhoea. Histopathological changes observed in the duodenum and jejunum were characteristic, with shedding of mucosal epithelial cells. In addition, the virus was detected in tissues of diarrhoea kids naturally infected with Aichivirus C, exhibiting pathological changes similar to those of experimental infections. Overall, this study first isolated Aichivirus C and confirmed its pathogenicity in kids, with further study needed to better understand the virus pathogenicity. As Aichivirus C has been detected in South Korea, Italy and the USA and widely prevalent in southwest China, the results obtained here have significant implications for the diagnosis and control of diarrhoea in goats.
摘要：
丙肝病毒C是山羊中的一种新兴病毒，但其生物学意义尚不清楚。在这项研究中,从四川省持续腹泻暴发的农场收集了18个腹泻和16个非腹泻的儿童粪便样本，2021年5月中国。在这些样本中，77.8%(14/18)的腹泻样本通过RT-PCR检测为丙肝病毒C阳性,显著高于非腹泻面部(0%，p<.001)；同时，在腹泻样本中未检测到山羊中其他常见的引起腹泻的病原体，除了两个被检测为山羊肠道病毒阳性的样本外，提示丙肝病毒与山羊腹泻有关。此外，使用Vero细胞系从阳性样品中成功分离出5株丙肝病毒C株，并进一步纯化了2株分离株，命名为SWUN/F5/2021（10-6.7TCID50/0.1mL）和SWUN/F6/2021（10-7TCID50/0.1mL）。有趣的是,丙肝病毒C株可通过口服给药在实验儿童中引起全身感染，主要临床表现为严重的水样腹泻。在十二指肠和空肠观察到的组织病理学变化是特征性的，粘膜上皮细胞脱落。此外,该病毒在自然感染丙肝病毒的腹泻儿童组织中检测到，表现出与实验性感染相似的病理变化。总的来说,本研究首次分离出丙肝病毒C，并证实其在儿童中的致病性，随着进一步的研究需要更好地了解病毒的致病性。由于在韩国已检测到丙肝病毒C,意大利和美国，在中国西南部广泛流行，本文获得的结果对山羊腹泻的诊断和控制具有重要意义。
