PDF(Pubmed)
Abstract：
Resistant wound microorganisms are becoming an extremely serious challenge in the process of treating infected chronic wounds, leading to impaired healing. Thus, additional approaches should be taken into consideration to improve the healing process. The use of natural extracts can represent a valid alternative to treat/control the microbial infections in wounds. This study investigates the antimicrobial/antivirulence effects of Capparis spinose aqueous extract against the main chronic wound pathogens: Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. The extract shows phenolic characterization with rutin (1.8 ± 0.14 μg/mg) as the major compound and antibacterial effect against bacteria (S. aureus PECHA 10 MIC 6.25%; P. aeruginosa PECHA 4 MIC 12.50%) without action against C. albicans (MIC and MFC ≥ 50%). Capparis spinose also shows a significant antivirulence effect in terms of antimotility/antibiofilm actions. In particular, the extract acts (i) on P. aeruginosa both increasing its swimming and swarming motility favoring the planktonic phenotype and reducing its adhesive capability, (ii) on S. aureus and P. aeruginosa biofilm formation reducing both the biomass and CFU/ml. Furthermore, the extract significantly displays the reduction of a dual-species S. aureus and P. aeruginosa Lubbock chronic wound biofilm, a complex model that mimics the realistic in vivo microbial spatial distribution in wounds. The results suggest that C. spinose aqueous extract could represent an innovative eco-friendly strategy to prevent/control the wound microbial infection.
摘要：
抗性伤口微生物在治疗感染的慢性伤口过程中成为一个极其严峻的挑战,导致愈合受损。因此，应考虑其他方法来改善愈合过程。使用天然提取物可以代表治疗/控制伤口中的微生物感染的有效替代方案。本研究调查了卡帕丽斯刺糖水提取物对主要慢性伤口病原体：金黄色葡萄球菌，铜绿假单胞菌,和白色念珠菌.提取物显示了以芦丁（1.8±0.14μg/mg）为主要化合物的酚类特征和对细菌的抗菌作用（S.金黄色葡萄球菌PECHA10MIC6.25％；铜绿假单胞菌PECHA4MIC12.50％）对白色念珠菌无作用（MIC和MFC≥50％）。就抗运动性/抗生物膜作用而言，卡帕里斯刺糖也显示出明显的抗毒作用。特别是,提取物（i）作用于铜绿假单胞菌，增加其游泳和成群运动，有利于浮游表型，并降低其粘附能力，(ii)在金黄色葡萄球菌和铜绿假单胞菌上形成的生物膜减少了生物量和CFU/ml。此外，提取物显着显示双物种金黄色葡萄球菌和铜绿假单胞菌Lubbock慢性伤口生物膜的减少，模拟伤口中现实体内微生物空间分布的复杂模型。结果表明,C.spinose水提取物可以代表预防/控制伤口微生物感染的创新的生态友好策略。
