Abstract：
The plerocercoid larvae of the tapeworm Spirometra erinaceieuropaei can parasitize humans and animals and cause serious parasitic zoonosis. However, our knowledge of the developmental process of S. erinaceieuropaei is still inadequate. To better characterize differential and specific genes and pathways associated with parasite development, a comparative transcriptomic analysis of the plerocercoid stage and the adult stage was performed using RNA-seq and de novo analysis. Approximately 13,659 differentially expressed genes (DEGs) were identified in plerocercoids versus adults, of which 6455 DEGs were upregulated and 7204 were downregulated. DEGs involved in parasite immunoevasion were more active in plerocercoid larvae than in adults, while DEGs associated with metabolic activity were upregulated in adults. Gene Ontology (GO) and Kyoto Encyclopedia of Genes (KEGG) analyses revealed that most DEGs involved in protein phosphorylation/dephosphorylation and the Wnt signalling pathway were much more active in plerocercoid larvae. The molecular functions of upregulated unigenes in adults were mainly enriched for metabolic activities. qPCR validated that the expression levels of 10 selected DEGs were consistent with those in RNA-seq, confirming the accuracy of the RNA-seq results. Our results contributed to increasing the knowledge on the S. erinaceieuropaei gene repertoire and expression profile and also provide valuable resources for functional studies on the molecular mechanisms of S. erinaceieuropaei.
摘要：
tape虫Spirometra虫的类虫幼虫可寄生于人类和动物，并引起严重的寄生虫人畜共患病。然而,我们对S.erinaceuropaei的发育过程的了解仍然不足。为了更好地表征与寄生虫发育相关的差异和特异性基因和途径，使用RNA-seq和从头分析,对plerocercoid期和成虫期进行了比较转录组学分析.大约13,659个差异表达基因（DEG）在plerocercoids与成人中被鉴定，其中6455个DEG上调，7204个下调。参与寄生虫免疫逃避的DEGs在plerocercoid幼虫中比在成虫中更活跃，而与代谢活动相关的DEGs在成人中上调。基因本体论（GO）和京都基因百科全书（KEGG）分析显示，参与蛋白质磷酸化/去磷酸化和Wnt信号通路的大多数DEGs在plerocercoid幼虫中更具活性。成人中上调的单基因的分子功能主要富集在代谢活性上。qPCR验证了10个选定的DEGs的表达水平与RNA-seq中的表达水平一致，确认RNA-seq结果的准确性。我们的结果有助于增加对S.erinaceuropaei基因库和表达谱的了解，也为S.erinaceuropaei的分子机制的功能研究提供了宝贵的资源。
