Abstract：
Phosphatidylinositol lipids regulate key processes, including vesicle trafficking and cell polarity. A recent study identified novel roles for phosphatidylinositol 4-phosphate (PI4P) in the plasma membrane of the fungal pathogen Candida albicans, including polarized hyphal growth and cell wall organization. Studies in other organisms were not able to separate the roles of PI4P in the plasma membrane and Golgi, but the C. albicans plasma membrane pool of PI4P could be selectively eliminated by deleting the STT4 kinase, which creates PI4P. Interestingly, stt4Δ mutants were strongly defective in disseminated candidiasis in mice but were not defective in an oral infection. This suggested that abnormal exposure of β-glucan in the mutant cell walls increased recruitment of innate immune cells during disseminated infection, which is not expected to impact oral infection. These results highlight novel roles of PI4P and reinforce the need to test the virulence of C. albicans mutants at different host sites.
摘要：
磷脂酰肌醇脂质调节关键过程，包括囊泡运输和细胞极性。最近的一项研究确定了磷脂酰肌醇4-磷酸（PI4P）在真菌病原体白色念珠菌的质膜中的新作用，包括极化菌丝生长和细胞壁组织。在其他生物体中的研究无法分离PI4P在质膜和高尔基体中的作用，但是通过删除STT4激酶可以选择性地消除PI4P的白色念珠菌质膜池，创建PI4P。有趣的是,stt4Δ突变体在小鼠的播散性念珠菌病中存在强烈缺陷，但在口腔感染中没有缺陷。这表明β-葡聚糖在突变细胞壁中的异常暴露增加了在播散性感染期间天然免疫细胞的募集。预计不会影响口腔感染。这些结果突出了PI4P的新作用,并且加强了在不同宿主位点测试白色念珠菌突变体的毒力的需要。
