PDF(Pubmed)
Abstract：
The internalization of apoptotic cells by non-phagocytic cells has been observed in different tissues and could be an important mechanism for the elimination of dying cells. Here, we describe a probable event of phagocytosis of apoptotic cells mediated by urothelial cells in urinary sediment. A 90-years-old male patient was admitted unconscious to the hospital, visible signs included: pale skin and dry mucous membranes, presumptively diagnosed as dehydration. Blood test revealed anaemia (haemoglobin 130 g/L) and hyperglycaemia (glucose 7.8 mmol/L), urinalysis showed a picture of urinary tract infection (leukocyturia and bacteriuria). The microscopic analysis of urinary sediment revealed the presence of urothelial cells and leukocytes internalized in urothelial cells. Anti-CD68 (membrane marker of macrophages) was tested by immunocytochemistry and a negative result was observed. Based on the findings phagocytosis of apoptotic cells mediated by urothelial cells was identified. This phenomenon can be observed in urinary sediment and should not be confused with a neoplastic process since it is a physiological event of cell elimination.
摘要：
已在不同组织中观察到非吞噬细胞对凋亡细胞的内化，这可能是消除垂死细胞的重要机制。这里,我们描述了尿沉渣中尿路上皮细胞介导的凋亡细胞吞噬的可能事件。一名90岁的男性患者昏迷入院，可见体征包括：皮肤苍白和粘膜干燥，被推定为脱水.血液检查显示贫血（血红蛋白130g/L）和高血糖（葡萄糖7.8mmol/L），尿液分析显示尿路感染（白细胞增多和细菌尿）。尿沉渣的显微镜分析显示存在尿路上皮细胞和内化在尿路上皮细胞中的白细胞。通过免疫细胞化学测试抗CD68(巨噬细胞的膜标记物)并且观察到阴性结果。基于该发现,鉴定了由尿路上皮细胞介导的凋亡细胞的吞噬作用。这种现象可以在尿沉渣中观察到，不应与肿瘤过程相混淆，因为它是细胞消除的生理事件。
