Abstract：
The abuse of antibiotics in aquaculture has led to the increasing rate of antibiotic resistance of aquatic bacteria including Aeromonas, which is an increasing threat to environmental and human health. To date, no epidemiological cut-off values (COWT) for Aeromonas spp. have been established by the Clinical and Laboratory Standards Institute nor the European Commission on Antimicrobial Susceptibility Testing. In this study, commercially prepared minimum inhibitory concentration (MIC) test 96-well plates (dry-form plates) were used to determine the MIC of eight antimicrobial agents against 556 Aeromonas strains. The obtained MIC distributions were simulated and analyzed by NRI and ECOFFinder to obtain tentative COWT values for Aeromonas spp. The COWT values of eight kinds of representative antimicrobial agents including trimethoprim-sulfamethoxazole, erythromycin, doxycycline, neomycin, colistin, florfenicol, enrofloxacin, and ceftazidime for Aeromonas spp. were established and were 0.25, 64/32, 4/2, 8, 4, 1, 0.062/0.125, and 0.5 μg/mL, respectively. Results showed that Aeromonas spp. had a very high proportion of non-wild-type strains to enrofloxacin, florfenicol, and doxycycline, which are the most widely used antimicrobials in aquaculture. The COWT values for Aeromonas spp. obtained in this study can contribute to the final establishment of COWT for Aeromonas spp. internationally.
摘要：
抗生素在水产养殖中的滥用导致包括气单胞菌在内的水生细菌对抗生素的耐药性增加。这对环境和人类健康的威胁越来越大。迄今为止,气单胞菌属无流行病学临界值（COWT）。由临床和实验室标准研究所或欧洲抗菌药物敏感性测试委员会建立。在这项研究中,商业制备的最低抑制浓度（MIC）测试96孔板（干板）用于确定八种抗微生物剂对556个气单胞菌菌株的MIC。通过NRI和ECOFFinder模拟和分析获得的MIC分布，以获得气单胞菌属的暂定COWT值。甲氧苄啶-磺胺甲恶唑等8种代表性抗菌药物的COWT值,红霉素,多西环素,新霉素,粘菌素,氟苯尼考,恩诺沙星,和头孢他啶用于气单胞菌属。分别为0.25、64/32、4/2、8、4、1、0.062/0.125和0.5μg/mL，分别。结果表明，气单胞菌属。恩诺沙星的非野生型菌株比例很高，氟苯尼考,和强力霉素,这是水产养殖中使用最广泛的抗菌剂。气单胞菌属的COWT值。在这项研究中获得的可有助于最终建立气单胞菌属的COWT。国际上。
