Abstract:
This study aimed to investigate whether different doses of specific phenolic acids (chlorogenic acid, caffeic acid, ferulic acid, gallic acid, protocatechuic acid, p-hydroxybenzoic acid, syringic acid, vanillic acid, and p-coumaric acid), alone or in combination, can inhibit the early growth of the common ragweed (Ambrosia artemisiifolia L., Asterales: Asteraceae). A seed bioassay was performed in Petri dishes and placed in a climate chamber to assess the effects of five dose levels of phenolic acids to radicle and shoot length, as well seedling biomass of A. artemisiifolia. The lowest dose of phenolic acid corresponded to the natural phenolic acid concentration previously reported in dry plant tissue samples from Brassicaceae cover crop plants. Results show that the inhibition of the early growth of A. artemisiifolia depends strongly on phenolic acid. Across different treatments, high doses of phenolic acids significantly shortened shoots and radicles, as well as reduced seedling biomass. Treating seeds with ferulic acid alone, vanillic acid alone, p-hydroxybenzoic acid alone, or a mixture of all phenolic acids significantly reduced all early growth parameters. The estimated effective dose for the 50% inhibition (ED50) of radicle growth in A. artemisiifolia seedlings was 368.39 ± 59.85 × 10-8 mol with ferulic acid, 135.41 ± 17.65 × 10-8 mol with p-coumaric acid, 810.36 ± 134.15 × 10-8 mol with p-hydroxybenzoic acid, and 160.11 ± 12.30 × 10-8 mol with the combination of all phenolic acids.
摘要:
本研究旨在探讨不同剂量的特定酚酸(绿原酸,咖啡酸,阿魏酸,没食子酸,原儿茶酸,对羟基苯甲酸,丁香酸,香草酸,和对香豆酸),单独或组合,可以抑制普通参草的早期生长(AmbrosiaartemisiifoliaL.,菊科:菊科)。在培养皿中进行种子生物测定,并放置在气候室中,以评估五种剂量水平的酚酸对胚根和芽长度的影响。以及青蒿幼苗生物量。酚酸的最低剂量对应于先前报道的来自十字花科覆盖作物的干植物组织样品中的天然酚酸浓度。结果表明,对青蒿的早期生长的抑制作用强烈依赖于酚酸。在不同的治疗方法中,高剂量的酚酸显着缩短了芽和胚根,以及减少幼苗生物量。用阿魏酸单独处理种子,单独的香草酸,单独使用对羟基苯甲酸,或所有酚酸的混合物显着降低了所有早期生长参数。阿魏酸对青蒿幼苗胚根生长的50%抑制(ED50)的估计有效剂量为368.39±59.85×10-8mol,135.41±17.65×10-8mol与对香豆酸,810.36±134.15×10-8mol对羟基苯甲酸,和160.11±12.30×10-8mol,所有酚酸的组合。