Abstract：
The study aimed to propose an efficient and eco-friendly strategy to improve the utilization of feather waste and converting it into high-valued antimicrobial products. Under the synergistic effect of instant catapult steam explosion (ICSE) (1.5 MPa-120 s), over 90% of chicken feather powder (CFP) was degraded into soluble peptides via keratinolysis within 3 h, about 90% of which were smaller than 3 kDa, indicating an overwhelming advantage than general proteolysis. Importantly, the keratinolysis hydrolysate of CFP was able to inhibit E. coli growth, among which the fraction < 3 kDa exhibited highest antimicrobial activity with a minimal inhibitory concentration of 30 mg/mL. Compared to other fractions, the fraction < 3 kDa contained higher content of hydrophobic amino acids (364.11 mg/g), in which about 79% of peptides had more than 60% hydrophobic ratio, potentially contributing to its antimicrobial activity. ICSE-keratinolysis process holds potential in reducing both protein resource waste and environmental pollution by valorizing feathers into antimicrobial product.
摘要：
该研究旨在提出一种高效和生态友好的策略，以提高羽毛废料的利用率并将其转化为高价值的抗菌产品。在瞬时弹射器蒸汽爆炸（ICSE）（1.5MPa-120s）的协同作用下，超过90%的鸡毛粉(CFP)在3小时内通过角质分解降解成可溶性肽,其中约90%小于3kDa，表明比一般蛋白水解具有压倒性优势。重要的是,CFP的角化水解产物能够抑制大肠杆菌的生长，其中<3kDa的级分表现出最高的抗微生物活性,最小抑制浓度为30mg/mL。与其他分数相比，<3kDa的部分含有较高含量的疏水性氨基酸（364.11mg/g），其中约79％的肽具有超过60％的疏水比，可能有助于其抗微生物活性。ICSE-角质分解工艺具有通过将羽毛增值为抗菌产品来减少蛋白质资源浪费和环境污染的潜力。
