PDF(Pubmed)
Abstract：
Dengchuan cattle are the only dairy yellow cattle and endangered cattle among Yunnan native cattle breeds. However, its genetic background remains unclear. Here, we performed whole-genome sequencing of ten Dengchuan cattle. Integrating our data with the publicly available data, Dengchuan cattle were observed to be highly interbred than other cattle in the dataset. Furthermore, the positive selective signals were mainly manifested in candidate genes and pathways related to milk production, disease resistance, growth and development, and heat tolerance. Notably, five genes (KRT39, PGR, KRT40, ESR2, and PRKACB) were significantly enriched in the estrogen signaling pathway. Moreover, the missense mutation in the PGR gene (c.190T > C, p.Ser64Pro) showed a homozygous mutation pattern with higher frequency (83.3%) in Dengchuan cattle. In addition, a large number of strong candidate regions matched genes and QTLs related to milk yield and composition. Our research provides a theoretical basis for analyzing the genetic mechanism underlying Dengchuan cattle with excellent lactation and adaptability, crude feed tolerance, good immune performance, and small body size and also laid a foundation for genetic breeding research of Dengchuan cattle in the future.
摘要：
登川牛是云南本地牛品种中唯一的奶牛黄牛和濒危牛。然而,其遗传背景尚不清楚。这里,我们对十头登川牛进行了全基因组测序。将我们的数据与公开数据整合，在数据集中观察到登川牛比其他牛具有高度的杂交能力。此外，阳性选择信号主要表现在与产奶相关的候选基因和途径中，抗病性,成长和发展，和耐热性。值得注意的是,五个基因(KRT39，PGR，KRT40、ESR2和PRKACB）在雌激素信号通路中显著富集。此外，PGR基因的错义突变(c.190T>C，p.Ser64Pro)在登川牛中表现出频率较高(83.3%)的纯合突变模式。此外,大量与产奶量和成分相关的强候选区域匹配基因和QTL。本研究为分析邓川牛泌乳和适应性优良的遗传机制提供了理论依据,粗饲料耐受性，良好的免疫性能，体型小，也为今后登川牛的遗传育种研究奠定了基础。
