PDF(Pubmed)
Abstract：
UNASSIGNED: The prime factor in determining the success of reimplantation of an avulsed tooth is the maintenance of the viability of periodontal ligament fibroblast cells (PDFC). This study aims to evaluate and compare Mc Carey Kaufman media (MK), Cornisol, Dulbecco\'s Modified Eagles Medium (DMEM), Hanks Balanced Salt Solution (HBSS) and distilled water in preserving the viability of the PDFC using the Cell Counting Kit-8 assay (CCK-8).
UNASSIGNED: Cryopreserved PDFC were suspended in DMEM and incubated in CO2 incubator at 370C with 95% humidity and 5% CO2 for attachment. Once cells attained 80% confluence, they were trypsinised and passed into T-25 culture flasks to expand the culture population. Cells from passage 5 were pooled for experimentation. Trypan blue exclusion test was performed before each experiment to measure cell viability and batches showing more than 95% viability were used in the experiment. The viable PDFC with 1×105 were seeded in 96 well plates and incubated in CO2 incubator at 370C, 95% humidity and 5% CO2 for 24 hours to allow cell attachment. A 100µL of the experimental media were added in the wells and the cells were exposed for 1, 24 and 48 hours respectively. The viability was determined using the CCK-8. Experiment was performed in triplicates and data was subjected to statistical analysis.
UNASSIGNED: Statistical analysis was performed using repeated measure ANOVA, ANOVA, and post-hoc Bonferroni test with the significance level p<0.05. The values are as follows: MK (1.3146 ±0.0588, 1.9012±0.0511, 2.0723±0.1211) > Cornisol (1.2399±0.0548, 1.9596±0.0652, 1.9592±0.1361) >DMEM (1.1914±0.0691, 1.8479±0.0116, 2.0718±0.0795) > HBSS (0.3665±0.0814, 0.0184±0.0010, 0.0248±0.0042) >distilled water (0.0122±0.0033, 0.0225±0.0085, 0.0104±0.0008) at 1 hour, 24 hours and 48 hours respectively. MK >Cornisol>DMEM>HBSS>distilled water.
UNASSIGNED: It can be concluded that the corneal preservation solutions showed promising results in preserving periodontal ligament cell viability for extended time periods.
UNASSIGNED: Cryopreserved PDFC were suspended in DMEM and incubated in CO2 incubator at 370C with 95% humidity and 5% CO2 for attachment. Once cells attained 80% confluence, they were trypsinised and passed into T-25 culture flasks to expand the culture population. Cells from passage 5 were pooled for experimentation. Trypan blue exclusion test was performed before each experiment to measure cell viability and batches showing more than 95% viability were used in the experiment. The viable PDFC with 1×105 were seeded in 96 well plates and incubated in CO2 incubator at 370C, 95% humidity and 5% CO2 for 24 hours to allow cell attachment. A 100µL of the experimental media were added in the wells and the cells were exposed for 1, 24 and 48 hours respectively. The viability was determined using the CCK-8. Experiment was performed in triplicates and data was subjected to statistical analysis.
UNASSIGNED: Statistical analysis was performed using repeated measure ANOVA, ANOVA, and post-hoc Bonferroni test with the significance level p<0.05. The values are as follows: MK (1.3146 ±0.0588, 1.9012±0.0511, 2.0723±0.1211) > Cornisol (1.2399±0.0548, 1.9596±0.0652, 1.9592±0.1361) >DMEM (1.1914±0.0691, 1.8479±0.0116, 2.0718±0.0795) > HBSS (0.3665±0.0814, 0.0184±0.0010, 0.0248±0.0042) >distilled water (0.0122±0.0033, 0.0225±0.0085, 0.0104±0.0008) at 1 hour, 24 hours and 48 hours respectively. MK >Cornisol>DMEM>HBSS>distilled water.
UNASSIGNED: It can be concluded that the corneal preservation solutions showed promising results in preserving periodontal ligament cell viability for extended time periods.
摘要：
未经证实：决定撕脱牙齿再植入成功的主要因素是维持牙周膜成纤维细胞（PDFC）的活力。本研究旨在评估和比较McCareyKaufman媒体(MK)，Cornisol,杜尔贝科改良老鹰培养基(DMEM)，Hanks平衡盐溶液(HBSS)和蒸馏水使用细胞计数试剂盒-8测定(CCK-8)保持PDFC的活力。
UNASSIGNED:将冷冻保存的PDFC悬浮在DMEM中,并在370°C、95%湿度和5%CO2的CO2培养箱中孵育用于附着。一旦细胞达到80%汇合,将它们胰蛋白酶化并传递到T-25培养瓶中以扩大培养种群。汇集来自第5代的细胞用于实验。在每个实验之前进行台盼蓝排除测试以测量细胞活力,并且在实验中使用显示超过95%活力的批次。将具有1×105的活PDFC接种在96孔板中，并在370℃的CO2培养箱中孵育，95%湿度和5%CO2持续24小时以允许细胞附着。在孔中加入100μL实验培养基,细胞分别暴露1、24和48小时。使用CCK-8测定生存力。一式三份进行实验,并对数据进行统计分析。
未经评估：使用重复测量方差分析进行统计分析，方差分析,和事后Bonferroni检验，显著性水平p<0.05。值如下：MK（1.3146±0.0588，1.9012±0.0511，2.0723±0.1211）>Cornisol（1.2399±0.0548，1.9596±0.0652，1.9592±0.1361）>DMEM（1.1914±0.0691，1.8479±0.0116，2.0718±0.0795）>0.00SS（0.3665±0.084，0.0082±0.085分别为24小时和48小时。MK>山梨醇>DMEM>HBSS>蒸馏水。
UNASSIGNED：可以得出结论，角膜保存溶液在延长的时间段内保存牙周膜细胞活力方面显示出有希望的结果。
UNASSIGNED:将冷冻保存的PDFC悬浮在DMEM中,并在370°C、95%湿度和5%CO2的CO2培养箱中孵育用于附着。一旦细胞达到80%汇合,将它们胰蛋白酶化并传递到T-25培养瓶中以扩大培养种群。汇集来自第5代的细胞用于实验。在每个实验之前进行台盼蓝排除测试以测量细胞活力,并且在实验中使用显示超过95%活力的批次。将具有1×105的活PDFC接种在96孔板中，并在370℃的CO2培养箱中孵育，95%湿度和5%CO2持续24小时以允许细胞附着。在孔中加入100μL实验培养基,细胞分别暴露1、24和48小时。使用CCK-8测定生存力。一式三份进行实验,并对数据进行统计分析。
未经评估：使用重复测量方差分析进行统计分析，方差分析,和事后Bonferroni检验，显著性水平p<0.05。值如下：MK（1.3146±0.0588，1.9012±0.0511，2.0723±0.1211）>Cornisol（1.2399±0.0548，1.9596±0.0652，1.9592±0.1361）>DMEM（1.1914±0.0691，1.8479±0.0116，2.0718±0.0795）>0.00SS（0.3665±0.084，0.0082±0.085分别为24小时和48小时。MK>山梨醇>DMEM>HBSS>蒸馏水。
UNASSIGNED：可以得出结论，角膜保存溶液在延长的时间段内保存牙周膜细胞活力方面显示出有希望的结果。
