PDF(Pubmed)
Abstract：
The study of the Mucoralean fungi physiology is a neglected field that the lack of effective genetic tools has hampered in the past. However, the emerging fungal infection caused by these fungi, known as mucormycosis, has prompted many researchers to study the pathogenic potential of Mucorales. The main reasons for this current attraction to study mucormycosis are its high lethality, the lack of effective antifungal drugs, and its recent increased incidence. The most contemporary example of the emergence character of mucormycosis is the epidemics declared in several Asian countries as a direct consequence of the COVID-19 pandemic. Fortunately, this pressure to understand mucormycosis and develop new treatment strategies has encouraged the blossoming of new genetic techniques and methodologies. This review describes the history of genetic manipulation in Mucorales, highlighting the development of methods and how they allowed the main genetic studies in these fungi. Moreover, we have emphasized the recent development of new genetic models to study mucormycosis, a landmark in the field that will configure future research related to this disease.
摘要：
Mucoralean真菌生理学的研究是一个被忽视的领域，过去缺乏有效的遗传工具阻碍了这一领域。然而,由这些真菌引起的新出现的真菌感染，被称为毛霉菌病，促使许多研究人员研究了Mucorales的致病潜力。目前这种吸引人研究毛霉菌病的主要原因是其高杀伤力，缺乏有效的抗真菌药物，以及最近增加的发病率。毛霉菌病出现特征的最当代的例子是几个亚洲国家宣布的流行病，这是COVID-19大流行的直接后果。幸运的是,了解毛霉菌病和开发新治疗策略的压力促使新的遗传技术和方法的兴起。这篇综述描述了Mucorales基因操作的历史，强调方法的发展以及它们如何允许这些真菌的主要遗传研究。此外，我们强调了最近研究毛霉菌病的新遗传模型的发展，这是该领域的一个里程碑，将配置与这种疾病有关的未来研究。
