PDF(Pubmed)
Abstract：
IR Biotyper (IRBT), which is a spectroscopic system for microorganism typing based on Fourier transform infrared (FTIR) technology, has been used to detect the spread of clones in clinical microbiology laboratories. However, the use of IRBT to detect probiotics has rarely been reported. Herein, we evaluated the discriminatory power of IRBT to type Lactiplantibacillus plantarum isolates at the strain level and explored its application potential in probiotic preliminary selection. Twenty Lactiplantibacillus isolates collected from pickled radishes during successive fermentation were used to test the robustness of IRBT at the strain level. IRBT was then compared with genotyping methods such as whole-genome sequencing (WGS), pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) to evaluate its discrimination power. IRBT distributed the 20 isolates into five clusters, with L. argentoratensis isolate C7-83 being the most distant from the other isolates, which belonged to L. plantarum. IRBT showed good reproducibility, although deviation in the discriminative power of IRBT was found at the strain level across laboratories, probably due to technical variance. All examined methods allowed bacterial identification at the strain level, but IRBT had higher discriminatory power than MLST and was comparable to the WGS and PFGE. In the phenotypic comparison study, we observed that the clustering results of probiotic physiological attributes (e.g., sensitivity to acid and bile salts, hydrophobicity of the cell surface, and resistance to antibiotics) were consistent with the typing results of IRBT. Our results indicated that IRBT is a robust tool for L. plantarum strain typing that could improve the efficiency of probiotic identification and preliminary screening, and can potentially be applied in probiotic traceability and quality control.
摘要：
红外生物分型仪(IRBT),这是一个基于傅里叶变换红外（FTIR）技术的微生物分型光谱系统，已用于检测临床微生物实验室中克隆的传播。然而,使用IRBT检测益生菌的报道很少.在这里，我们在菌株水平上评估了IRBT对植物乳杆菌分离株的判别能力，并探讨了其在益生菌初步选择中的应用潜力。在连续发酵过程中从腌制的萝卜中收集的二十种肌醇杆菌分离株用于测试IRBT在菌株水平上的稳健性。然后将IRBT与基因分型方法进行比较，如全基因组测序（WGS），脉冲场凝胶电泳(PFGE),和多位点序列分型(MLST)来评价其辨别力。IRBT将20个分离株分成五个簇，与L.argentoratensis分离株C7-83是最远离其他分离株，属于植物乳杆菌。IRBT表现出良好的重现性，尽管在实验室的应变水平上发现了IRBT的辨别能力的偏差，可能是由于技术差异。所有检查过的方法都允许在菌株水平上进行细菌鉴定，但IRBT的歧视能力高于MLST，与WGS和PFGE相当。在表型比较研究中，我们观察到益生菌生理属性的聚类结果(例如，对酸和胆盐的敏感性，细胞表面的疏水性，和对抗生素的耐药性）与IRBT的分型结果一致。我们的结果表明，IRBT是植物乳杆菌菌株分型的强大工具，可以提高益生菌鉴定和初步筛选的效率，并有可能应用于益生菌的可追溯性和质量控制。
