Abstract：
Phage are thought to exhibit control over host genes during infection. As a preliminary investigation of the kinetics and magnitude of co-expression between phage and bacteria, we compared the global transcriptional profiles for Vibrio alginolyticus strain E110 and its lytic phage HH109 by using RNA sequencing. In total, 24.7% (1,143/4,620) of the host protein-coding genes were differentially expressed genes during infection (DEGs). Functional analysis of the host DEGs suggests that phage HH109 induced rapid and distinctive changes when compared with 60- and 120-min postinfection (mpi). Based on gene co-expression network analysis, an uncharacterized late gene gp27 encoded by the phage HH109 was predicted to modulate the host\'s membrane transport and/or transcriptional regulation. Furthermore, expression of several bacterial virulence genes was downregulated while drug resistance genes were upregulated. This work contributes to an in-depth understanding of the reciprocal interactions of lytic phage HH109 and its pathogenic Vibrio host E110, and can provide new insights into the research and development of phage therapy against pathogenic Vibrio infections in the economically significant aquatic animals. IMPORTANCE Vibrio alginolyticus is a common opportunistic pathogen that causes mass mortality in cultured marine animals. Phage HH109 lyses pathogenic V. alginolyticus strain E110 with high efficiency and thus serves as a useful model to understand the dynamic interplay of a phage and its host. Global transcriptomic responses of strain E110 post-HH109 infection were characterized by using RNA sequencing, elucidating step-by-step control by HH109, an antiphage-like responses, and the elevated expression of drug resistance. This study provides a detailed molecular description phage and V. alginolyticus, providing insight into better prevention and control of vibriosis in aquatic animals.
摘要：
噬菌体被认为在感染期间表现出对宿主基因的控制。作为噬菌体和细菌共表达的动力学和大小的初步研究，我们通过使用RNA测序比较了溶藻弧菌E110菌株及其裂解噬菌体HH109的全局转录谱。总的来说,24.7%(1,143/4,620)的宿主蛋白编码基因是感染期间差异表达的基因。宿主DEGs的功能分析表明，与感染后60分钟和120分钟（mpi）相比，噬菌体HH109诱导了快速而独特的变化。基于基因共表达网络分析,预测由噬菌体HH109编码的未表征的晚期基因gp27可以调节宿主的膜转运和/或转录调节。此外，几种细菌毒力基因表达下调，而耐药基因表达上调。这项工作有助于深入了解裂解性噬菌体HH109及其致病性弧菌宿主E110的相互作用，并可以为在具有经济意义的水生动物中针对致病性弧菌感染的噬菌体疗法的研究和开发提供新的见解。重要性溶藻弧菌是一种常见的机会病原体，可导致养殖海洋动物大量死亡。噬菌体HH109高效裂解致病性溶藻弧菌菌株E110，因此可作为了解噬菌体及其宿主动态相互作用的有用模型。HH109感染后E110菌株的全局转录组反应通过使用RNA测序来表征，阐明HH109的逐步控制，一种抗噬菌体样反应，以及耐药性的升高表达。本研究提供了详细的分子描述噬菌体和溶藻弧菌，为更好地预防和控制水生动物的弧菌病提供见解。
