PDF(Pubmed)
Abstract：
The aim of the present study was to determine whether the echotextural features of the mammary gland parenchyma in buffaloes during lactation at different somatic cell levels could be used to diagnose mastitis. This study was divided into two parts. In the first experiment, experimental buffaloes (n = 65) with somatic cell counts (SCC) tests (n = 94) in different seasons, including spring (n = 22), summer (n = 24), autumn (n = 37), and winter (n = 11), were used to obtain ultrasonic variables for each quarter of mammary gland that could best explain the corresponding somatic cell level. In the second part of the study, the first part\'s experimental results were verified by subjecting at least one-quarter udder of eight buffaloes to ultrasonography seven times during mid-July to mid-August for obtaining ultrasonic values at different somatic cell levels. The echo textural characteristics [mean numerical pixel values (NPVs) and pixel heterogeneity (pixel standard deviation, PSD)] were evaluated using 16 ultrasonographic images of each buffalo with Image ProPlus software. The effects of SCC, days in milk (DIM), scanning order (SO), season, as well as the scanning plane and udder quarter (SP + UQ) on both the PSD and NPVs of the mammary gland were significant (p < 0.05). The correlation coefficient between pre-milking sagittal PSD and somatic cell score (SCS) was the highest (r = 0.4224, p < 0.0001) with fitted linear model: y = 0.19445x (dependent variable: SCS, independent variables: pre-milking sagittal PSD; R 2 = 0.84, p < 0.0001). In addition, SCC and ultrasonic of udder quarter were followed for 1 month, confirming that pre-milking sagittal PSD of mammary gland value could explain the SCC variation in milk. The current study demonstrated that the ultrasonographic examination of the udder could be one of the complementary tools for diagnosing subclinical mastitis in buffaloes.
摘要：
本研究的目的是确定在不同体细胞水平下哺乳期水牛乳腺实质的回声特征是否可用于诊断乳腺炎。本研究分为两部分。在第一个实验中,实验水牛（n=65）在不同季节进行体细胞计数（SCC）测试（n=94），包括弹簧（n=22），夏季（n=24），秋天（n=37），和冬天（n=11），用于获得乳腺每个季度的超声变量，以最好地解释相应的体细胞水平。在研究的第二部分,第一部分的实验结果通过在7月中旬至8月中旬对8只水牛的至少四分之一乳房进行7次超声检查来验证，以获得不同体细胞水平的超声值。回声纹理特征[平均数值像素值(NPV)和像素异质性(像素标准偏差，PSD）]使用ImageProPlus软件使用每头水牛的16张超声图像进行评估。SCC的影响，牛奶中的天数（DIM），扫描顺序(SO)，季节,乳腺PSD和NPV的扫描平面和乳房四分之一（SPUQ）均显着（p<0.05）。挤奶前矢状PSD与体细胞评分（SCS）的相关系数最高（r=0.4224，p<0.0001），拟合线性模型为：y=0.19445x（因变量：SCS，独立变量：挤奶前矢状PSD；R2=0.84，p<0.0001）。此外,术后随访1个月，术后1个月，证实挤奶前乳腺矢状位PSD值可以解释乳汁中SCC的变化。目前的研究表明，乳房的超声检查可能是诊断水牛亚临床乳腺炎的补充工具之一。
