Abstract：
OBJECTIVE: Shellfish waste is a primary source for making N-acetyl-D-glucosamine. Thus, establishing a high-efficiency and low-cost bioconversion method to produce N-acetyl-D-glucosamine directly from shellfish waste was promising.
RESULTS: A mutant C81 was obtained from Chitinolyticbacter meiyuanensis SYBC-H1 via 60Co-γ irradiation. This mutant C81 showed the highest chitinase activity of 9.8 U/mL that was 85% higher than the parent strain. The mutant C81 exhibted improved antioxidant activities, including total antioxidant capacity, superoxide radical ability, and hydroxyl radical scavenging ability, compared to that of the parent strain. Four out of nine organic solvents increased the chitinase activity by 1.9%, 6.8%, 11.7%, and 15.8%, corresponding to methylbenzene, n-heptane, petroleum ether, and n-hexane, respectively. The biphase system composed of aqueous and hexane presented a five-fold reduction of cell viability compared to the control. Using a continuous fermentation bioconversion process, 4.2 g/L GlcNAc was produced from crayfish shell powder with a yield of 80% of the chitin content.
CONCLUSIONS: This study demonstrated that the mutant C81 is suitable for converting crayfish shell powder into GlcNAc in an aqueous-organic system.
RESULTS: A mutant C81 was obtained from Chitinolyticbacter meiyuanensis SYBC-H1 via 60Co-γ irradiation. This mutant C81 showed the highest chitinase activity of 9.8 U/mL that was 85% higher than the parent strain. The mutant C81 exhibted improved antioxidant activities, including total antioxidant capacity, superoxide radical ability, and hydroxyl radical scavenging ability, compared to that of the parent strain. Four out of nine organic solvents increased the chitinase activity by 1.9%, 6.8%, 11.7%, and 15.8%, corresponding to methylbenzene, n-heptane, petroleum ether, and n-hexane, respectively. The biphase system composed of aqueous and hexane presented a five-fold reduction of cell viability compared to the control. Using a continuous fermentation bioconversion process, 4.2 g/L GlcNAc was produced from crayfish shell powder with a yield of 80% of the chitin content.
CONCLUSIONS: This study demonstrated that the mutant C81 is suitable for converting crayfish shell powder into GlcNAc in an aqueous-organic system.
摘要：
目的：贝类废物是制造N-乙酰-D-氨基葡萄糖的主要来源。因此，建立一种高效，低成本的生物转化方法，直接从贝类废物中生产N-乙酰-D-氨基葡萄糖是有前途的。
结果：通过60Co-γ辐射从木质病菌SYBC-H1中获得突变体C81。该突变体C81显示出9.8U/mL的最高几丁质酶活性，比亲本菌株高85％。突变体C81表现出改善的抗氧化活性，包括总抗氧化能力，超氧自由基能力，和羟自由基清除能力，与亲本菌株相比。九种有机溶剂中有四种使几丁质酶活性提高了1.9％，6.8%,11.7%,和15.8%，对应于甲苯，正庚烷,石油醚,和正己烷,分别。与对照相比，由水溶液和己烷组成的双相系统使细胞活力降低了五倍。采用连续发酵生物转化工艺,4.2g/LGlcNAc是从小龙虾壳粉末生产的，产量为几丁质含量的80％。
结论：本研究表明，突变体C81适用于在水-有机体系中将小龙虾壳粉转化为GlcNAc。
结果：通过60Co-γ辐射从木质病菌SYBC-H1中获得突变体C81。该突变体C81显示出9.8U/mL的最高几丁质酶活性，比亲本菌株高85％。突变体C81表现出改善的抗氧化活性，包括总抗氧化能力，超氧自由基能力，和羟自由基清除能力，与亲本菌株相比。九种有机溶剂中有四种使几丁质酶活性提高了1.9％，6.8%,11.7%,和15.8%，对应于甲苯，正庚烷,石油醚,和正己烷,分别。与对照相比，由水溶液和己烷组成的双相系统使细胞活力降低了五倍。采用连续发酵生物转化工艺,4.2g/LGlcNAc是从小龙虾壳粉末生产的，产量为几丁质含量的80％。
结论：本研究表明，突变体C81适用于在水-有机体系中将小龙虾壳粉转化为GlcNAc。
