PDF(Pubmed)
Abstract：
In the current study, we synthesized nanocellulose (NCF)-collagen (Col)-nano hydroxyapatite (NHA) organic-inorganic hybrid aerogels loaded with stromal cell derived factor-1 (SDF-1) and sclerostin monoclonal antibody (SOST McAb) and investigated their ability to repair steroid-induced osteonecrosis. Rabbit bone marrow mesenchymal stem cells (BMSCs) and human vascular endothelial cells (HUVECs) were used for the in vitro study. A rabbit steroid-induced osteonecrosis model was used for the in vivo study. The best elastic modulus reached 12.95 ± 4.77 MPa with a mean compressive property of 0.4067 ± 0.084 MPa for the scaffold containing 100% mass fraction. The average pore diameter of the aerogel was 75 ± 18 µm with a porosity of more than 90% (96.4 ± 1.6%). The aerogel-loaded SDF-1 and SOST were released at 40-50% from the material within the initial 3 h and maintained a stable release for more than 21 days. The in vitro study showed osteogenesis and vascularization capabilities of the scaffold. The in vivo study showed that rabbits received implantation of the scaffold with SOST McAb and SDF-1 showed the best osteogenesis of the osteonecrosis zone in the femoral head. Imaging examination revealed that most of the necrotic area of the femoral head was repaired. These results suggest that this hybrid aerogel scaffold could be used for future steroid-induced osteonecrosis repair.
摘要：
在目前的研究中,我们合成了纳米纤维素（NCF）-胶原（Col）-纳米羟基磷灰石（NHA）有机-无机混合气凝胶，负载基质细胞衍生因子-1（SDF-1）和硬化蛋白单克隆抗体（SOSTMcAb），并研究了它们修复类固醇诱导的骨坏死的能力。兔骨髓间充质干细胞（BMSCs）和人血管内皮细胞（HUVECs）用于体外研究。兔类固醇诱导的骨坏死模型用于体内研究。质量分数为100％的支架，最佳弹性模量达到12.95±4.77MPa，平均压缩性能为0.4067±0.084MPa。气凝胶的平均孔径为75±18μm，孔隙率超过90％（96.4±1.6％）。加载气凝胶的SDF-1和SOST在最初3小时内从材料中释放40-50%,并保持稳定释放超过21天。体外研究显示了支架的成骨和血管形成能力。体内研究表明，兔接受SOSTMcAb和SDF-1的支架植入显示股骨头坏死区的最佳成骨作用。影像学检查显示股骨头坏死区大部分已修复。这些结果表明，这种混合气凝胶支架可用于未来的类固醇诱导的骨坏死修复。
