PDF(Pubmed)
Abstract：
Introduction: The egg shell membrane (ESM) is always considered as waste, but recent studies have shown that it has the potential to yield rapid re-endothelialization in vitro. We hypothesized that ESM and heparin-conjugated ESM (HESM) can be used as arterial patch in a rat aortic angioplasty model. Method: Sprague-Dawley rat (200 g) abdominal aortic patch angioplasty model was used. Decellularized rat thoracic aorta (TA) patch was used as the control; ESM patch was made of raw chicken egg; heparin-coated ESM (HESM) patch was made by using dopamine; anticoagulation properties were verified using platelet adhesion tests; the TA, ESM, and HESM patches were implanted to the rat aorta and harvested at day 14; and the samples were examined by immunohistochemistry and immunofluorescence. Result: The ESM patch showed a similar healing process to the TA patch; the cells could migrate and infiltrate into both patches; there was a neointima with von Willebrand factor-positive endothelial cells; the endothelial cells acquired arterial identity with Ephrin-B2- and dll-4-positive cells; there were proliferating cell nuclear antigen (PCNA)-positive cells, and PCNA and alpha smooth muscle actin dual-positive cells in the neointima in both groups. Heparin was conjugated to the patch successfully and showed a strong anticoagulation property in vitro. HESM could decrease mural thrombus formation after rat aortic patch angioplasty. Conclusion: The ESM is a natural scaffold that can be used as a vascular patch; it showed a similar healing process to decellularized TA patch; HESM showed anticoagulation property both in vitro and in vivo; and the ESM may be a promising vascular graft in the clinic.
摘要：
简介：蛋壳膜（ESM）总是被认为是废物，但最近的研究表明，它有可能在体外产生快速的再内皮化。我们假设ESM和肝素缀合的ESM（HESM）可以用作大鼠主动脉血管成形术模型中的动脉补片。方法:采用SD大鼠(200g)腹主动脉补片血管成形术模型。以脱细胞大鼠胸主动脉（TA）贴片为对照；ESM贴片由生鸡蛋制成；肝素涂层ESM（HESM）贴片通过使用多巴胺制成；使用血小板粘附试验验证抗凝性能；TA，ESM,将HESM贴片植入大鼠主动脉并在第14天收获;并通过免疫组织化学和免疫荧光检查样品。结果：ESM贴片显示与TA贴片相似的愈合过程；细胞可以迁移并浸润到两个贴片中；有一个新内膜，具有vonWillebrand因子阳性的内皮细胞；内皮细胞获得了与Ephrin-B2-和dll-4阳性细胞的动脉同一性；有增殖细胞核抗原（PCNA）阳性细胞，两组新生内膜中PCNA和α平滑肌肌动蛋白双阳性细胞。肝素与贴剂成功缀合，并在体外显示出强的抗凝特性。HESM可以减少大鼠主动脉补片血管成形术后的壁血栓形成。结论：ESM是一种天然支架，可作为血管补片，其愈合过程与脱细胞TA补片相似；HESM在体内和体外均具有抗凝性能；ESM可能是临床上很有前途的血管移植物。
