PDF(Pubmed)
Abstract：
In vitro production (IVP) embryos have a reduced quality and poor cryotolerance in comparison to in vivo embryos. This study investigated whether free fatty acid (FFA) conditions, fatty acid free (FAF)- synthetic oviduct fluid (SOF) without or with 25 μM of saturated stearic (C18:0) or unsaturated oleic (C18:1) acid during the first 5 IVP days, relate to quality and cryosurvival of day 8 blastocysts. Apart from the blastocyst scores, both 1) number and size of lipid droplets of fresh blastocysts and 2) total number and apoptotic and necrotic cells, before and after freezing-thawing, were scored by confocal microscopy. Blastocyst rates were significantly lower in the FAF SOF condition in comparison to other groups. Interestingly, blastocysts originating from the C18:1 group, with a significantly higher lipid content, and blastocysts from the FAF SOF group demonstrated a high cryosurvival rate (70.1 and 67.4%, respectively) comparable with in vivo blastocysts (68%), in contrast to the poor cryosurvival of C18:0 exposed embryos (17.6%). In all freeze-thawed embryos the average amount of apoptotic and necrotic cells increased albeit that the C18:0 condition rates were higher (43.2%) when compared to C18:1 (26.0%) and FAF SOF conditions (26.5%). The current data show that FFA administered during early embryonic development significantly affect the cryotolerance of blastocysts.
摘要：
与体内胚胎相比，体外生产（IVP）胚胎的质量降低且低温耐受性差。这项研究调查了游离脂肪酸(FFA)条件是否，无脂肪酸（FAF）-在前5个IVP天期间，不含或含25μM饱和硬脂酸（C18：0）或不饱和油酸（C18：1）的合成输卵管液（SOF），与第8天囊胚的质量和冷冻存活有关。除了胚泡评分,1)新鲜囊胚脂滴的数量和大小，2)总数和凋亡和坏死细胞，在冻融之前和之后，通过共聚焦显微镜进行评分。与其他组相比，FAFSOF条件下的囊胚率明显较低。有趣的是,来自C18：1组的胚泡，具有明显更高的脂质含量，FAFSOF组的囊胚表现出很高的冷冻存活率（70.1％和67.4％，分别)与体内胚泡(68%)相当，与C18：0暴露胚胎（17.6％）的冷冻存活率差相反。在所有冻融胚胎中，凋亡和坏死细胞的平均数量增加，尽管与C18：1（26.0％）和FAFSOF条件（26.5％）相比，C18：0条件的发生率更高（43.2％）。目前的数据显示,在早期胚胎发育期间施用的FFA显著影响胚泡的低温耐受性。
