UNASSIGNED: Conventional anal cytology was performed in 86 adult consenting subjects with history of receptive anal intercourse (RAI) and 4 controls without history of RAI. Cases with a-SIL and controls were subjected to fluorescent in-situ hybridization (FISH) to evaluate hTERT gene and chromosome 7 expression, as marker of cell ploidy. Results were expressed as number of abnormal nuclei (≥3 respective signals), maximum degree of amplification, mean signals/nucleus and proportion of cases showing abnormal nuclei.
UNASSIGNED: Twenty cases showed a-SIL; with 15 atypical squamous cells of undetermined significance (ASCUS), 3 low grade squamous intra-epithelial lesion (LSIL) and 2 cases of high-risk cytology. Expression of both hTERT gene and chromosome 7 increased from controls to ASCUS to LSIL with concomitant increase in proportion of cases having abnormal hTERT gene and chromosome 7 expression.
UNASSIGNED: Positive association of hTERT gene with a-SIL suggests its possible role in evolution of anal squamous abnormalities. Increase in chromosome 7 also correlated positively with a-SIL. These findings corroborate the similarities between squamous carcinogenesis in CIN and a-SIL.
UNASSIGNED:对86名有接受性肛门性交史(RAI)的成年同意受试者和4名无RAI史的对照进行了常规肛门细胞学检查。对具有a-SIL和对照的病例进行荧光原位杂交(FISH)以评估hTERT基因和7号染色体的表达,作为细胞倍性的标记。结果表示为异常核的数量(≥3个相应的信号),最大程度的放大,平均信号/细胞核和显示异常细胞核的病例比例。
UNASSIGNED:20例显示a-SIL;有15个意义不明的非典型鳞状细胞(ASCUS),低度鳞状上皮内病变(LSIL)3例,高危细胞学2例。hTERT基因和7号染色体的表达从对照到ASCUS再到LSIL都增加,同时hTERT基因和7号染色体表达异常的病例比例也随之增加。
UNASSIGNED:hTERT基因与a-SIL的正相关表明其可能在肛门鳞状异常的演变中发挥作用。染色体7的增加也与a-SIL正相关。这些发现证实了CIN和a-SIL鳞状细胞癌变之间的相似性。