PDF(Pubmed)
Abstract：
UNASSIGNED: Akin to cervical squamous intra-epithelial neoplasia (CIN), anal squamous intra-epithelial lesion (a-SIL) is attributed to persistent infection with high-risk human papilloma virus infection. Amplification of human telomerase reverse transcriptase (hTERT) gene and aneuploidy are known to correlate with CIN evolution. It is plausible that the underlying genetic events in a-SIL are similar. We conducted this cross-sectional analytical study with the objective of determining expression of hTERT gene expression and chromosome 7, as marker of cell ploidy in a-SIL.
UNASSIGNED: Conventional anal cytology was performed in 86 adult consenting subjects with history of receptive anal intercourse (RAI) and 4 controls without history of RAI. Cases with a-SIL and controls were subjected to fluorescent in-situ hybridization (FISH) to evaluate hTERT gene and chromosome 7 expression, as marker of cell ploidy. Results were expressed as number of abnormal nuclei (≥3 respective signals), maximum degree of amplification, mean signals/nucleus and proportion of cases showing abnormal nuclei.
UNASSIGNED: Twenty cases showed a-SIL; with 15 atypical squamous cells of undetermined significance (ASCUS), 3 low grade squamous intra-epithelial lesion (LSIL) and 2 cases of high-risk cytology. Expression of both hTERT gene and chromosome 7 increased from controls to ASCUS to LSIL with concomitant increase in proportion of cases having abnormal hTERT gene and chromosome 7 expression.
UNASSIGNED: Positive association of hTERT gene with a-SIL suggests its possible role in evolution of anal squamous abnormalities. Increase in chromosome 7 also correlated positively with a-SIL. These findings corroborate the similarities between squamous carcinogenesis in CIN and a-SIL.
UNASSIGNED: Conventional anal cytology was performed in 86 adult consenting subjects with history of receptive anal intercourse (RAI) and 4 controls without history of RAI. Cases with a-SIL and controls were subjected to fluorescent in-situ hybridization (FISH) to evaluate hTERT gene and chromosome 7 expression, as marker of cell ploidy. Results were expressed as number of abnormal nuclei (≥3 respective signals), maximum degree of amplification, mean signals/nucleus and proportion of cases showing abnormal nuclei.
UNASSIGNED: Twenty cases showed a-SIL; with 15 atypical squamous cells of undetermined significance (ASCUS), 3 low grade squamous intra-epithelial lesion (LSIL) and 2 cases of high-risk cytology. Expression of both hTERT gene and chromosome 7 increased from controls to ASCUS to LSIL with concomitant increase in proportion of cases having abnormal hTERT gene and chromosome 7 expression.
UNASSIGNED: Positive association of hTERT gene with a-SIL suggests its possible role in evolution of anal squamous abnormalities. Increase in chromosome 7 also correlated positively with a-SIL. These findings corroborate the similarities between squamous carcinogenesis in CIN and a-SIL.
摘要：
未经证实：类似于宫颈鳞状上皮内瘤变（CIN），肛门鳞状上皮内病变(a-SIL)是由高危型人乳头瘤病毒感染的持续性感染所致.已知人端粒酶逆转录酶（hTERT）基因的扩增和非整倍性与CIN进化相关。似乎a-SIL中的潜在遗传事件是相似的。我们进行了这项横断面分析研究，目的是确定hTERT基因表达和7号染色体的表达，作为a-SIL中细胞倍性的标志物。
UNASSIGNED：对86名有接受性肛门性交史（RAI）的成年同意受试者和4名无RAI史的对照进行了常规肛门细胞学检查。对具有a-SIL和对照的病例进行荧光原位杂交（FISH）以评估hTERT基因和7号染色体的表达，作为细胞倍性的标记。结果表示为异常核的数量（≥3个相应的信号），最大程度的放大，平均信号/细胞核和显示异常细胞核的病例比例。
UNASSIGNED：20例显示a-SIL；有15个意义不明的非典型鳞状细胞（ASCUS），低度鳞状上皮内病变（LSIL）3例，高危细胞学2例。hTERT基因和7号染色体的表达从对照到ASCUS再到LSIL都增加，同时hTERT基因和7号染色体表达异常的病例比例也随之增加。
UNASSIGNED：hTERT基因与a-SIL的正相关表明其可能在肛门鳞状异常的演变中发挥作用。染色体7的增加也与a-SIL正相关。这些发现证实了CIN和a-SIL鳞状细胞癌变之间的相似性。
UNASSIGNED：对86名有接受性肛门性交史（RAI）的成年同意受试者和4名无RAI史的对照进行了常规肛门细胞学检查。对具有a-SIL和对照的病例进行荧光原位杂交（FISH）以评估hTERT基因和7号染色体的表达，作为细胞倍性的标记。结果表示为异常核的数量（≥3个相应的信号），最大程度的放大，平均信号/细胞核和显示异常细胞核的病例比例。
UNASSIGNED：20例显示a-SIL；有15个意义不明的非典型鳞状细胞（ASCUS），低度鳞状上皮内病变（LSIL）3例，高危细胞学2例。hTERT基因和7号染色体的表达从对照到ASCUS再到LSIL都增加，同时hTERT基因和7号染色体表达异常的病例比例也随之增加。
UNASSIGNED：hTERT基因与a-SIL的正相关表明其可能在肛门鳞状异常的演变中发挥作用。染色体7的增加也与a-SIL正相关。这些发现证实了CIN和a-SIL鳞状细胞癌变之间的相似性。
