Abstract：
Numerous intervention strategies have been developed to promote functional tissue repair following experimental spinal cord injury (SCI), including the bridging of lesion-induced cystic cavities with bioengineered scaffolds. Integration between such implanted scaffolds and the lesioned host spinal cord is critical for supporting regenerative growth, but only moderate-to-low degrees of success have been reported. Light and electron microscopy were employed to better characterise the fibroadhesive scarring process taking place after implantation of a longitudinally microstructured type-I collagen scaffold into unilateral mid-cervical resection injuries of the adult rat spinal cord. At long survival times (10 weeks post-surgery), sheets of tightly packed cells (of uniform morphology) could be seen lining the inner surface of the repaired dura mater of lesion-only control animals, as well as forming a barrier along the implant-host interface of the scaffold-implanted animals. The highly uniform ultrastructural features of these scarring cells and their anatomical continuity with the local, reactive spinal nerve roots strongly suggest their identity to be perineurial-like cells. This novel aspect of the cellular composition of reactive spinal cord tissue highlights the increasingly complex nature of fibroadhesive scarring involved in traumatic injury, and particularly in response to the implantation of bioengineered collagen scaffolds.
摘要：
已经开发了许多干预策略来促进实验性脊髓损伤（SCI）后的功能性组织修复，包括用生物工程支架桥接病变诱导的囊腔。这种植入的支架和受损的宿主脊髓之间的整合对于支持再生生长至关重要。但只报道了中低的成功程度。采用光学和电子显微镜来更好地表征将纵向微结构的I型胶原支架植入成年大鼠脊髓的单侧中颈切除损伤后发生的纤维粘附性瘢痕形成过程。在长生存时间（手术后10周），可以看到紧密堆积的细胞（形态均匀）的片衬在仅有病变的对照动物的修复硬脑膜的内表面上，以及沿着支架植入动物的植入物-宿主界面形成屏障。这些瘢痕形成细胞的高度均匀的超微结构特征及其与局部的解剖学连续性，反应性脊神经根强烈表明它们是神经周样细胞。反应性脊髓组织细胞组成的这一新颖方面突出了与创伤性损伤有关的纤维粘附性瘢痕形成的日益复杂的性质。特别是响应生物工程胶原支架的植入。
