RESULTS: A total of 1720 and 6178 differentially expressed genes were identified from C. lanuginose and C. crassifolia, respectively. The photosynthesis and oxidation-reduction processes of C. crassifolia were more sensitive than C. lanuginose under heat stress. Glycine/serine/threonine metabolism, glyoxylic metabolism, and thiamine metabolism were important pathways in response to heat stress in C. lanuginose, and flavonoid biosynthesis, phenylalanine metabolism, and arginine/proline metabolism were the key pathways in C. crassifolia. Six sHSPs (c176964_g1, c200771_g1, c204924_g1, c199407_g2, c201522_g2, c192936_g1), POD1 (c200317_g1), POD3 (c210145_g2), DREB2 (c182557_g1), and HSFA2 (c206233_g2) may be key genes in the response to heat stress in C. lanuginose and C. crassifolia.
CONCLUSIONS: We compared important metabolic pathways and differentially expressed genes in response to heat stress between C. lanuginose and C. crassifolia. The results increase our understanding of the response mechanism and candidate genes of Clematis under heat stress. These data may contribute to the development of new Clematis varieties with greater heat tolerance.
结果:共鉴定出1720个和6178个差异表达基因。分别。在热胁迫下,青海三叶草的光合作用和氧化还原过程比三叶草糖更敏感。甘氨酸/丝氨酸/苏氨酸代谢,乙醛酸代谢,和硫胺素代谢是响应热应激的重要途径。和类黄酮生物合成,苯丙氨酸代谢,精氨酸/脯氨酸代谢是青海三叶草的关键途径。六个sHSP(c176964_g1,c200771_g1,c204924_g1,c199407_g2,c201522_g2,c192936_g1),POD1(c200317_g1),POD3(c210145_g2),DREB2(c182557_g1),和HSFA2(c206233_g2)可能是响应热胁迫的关键基因。
结论:我们比较了羊绿草糖和塞浦路斯C.cassifolia在热胁迫下的重要代谢途径和差异表达基因。该结果增加了我们对铁线莲在热应激下的反应机制和候选基因的理解。这些数据可能有助于开发具有更高耐热性的新铁线莲品种。