This study aimed to determine the features of bone metabolism in rats with lipopolysaccharide (LPS)-induced periodontitis combined with chronic thiolactone HHcy.
Forty-eight white, non-linear, mature rats were divided into 4 groups: control (n = 12); LPS‑induced periodontitis (n = 12); chronic thiolactone HHcy (n = 12); and periodontitis combined with HHcy (n = 12). The rats were sacrificed the day after the last LPS injection or the day after the last homocysteine (Hcy) thiolactone administration. Bone metabolism was determined based on the activity of alkaline phosphatase (ALP) and acid phosphatase (AP) in blood serum and periodontal homogenate.
A decrease in ALP activity (by 40.1%; р = 0.001) and the mineralization index (MI) (3.5 times; р < 0.001) with an increase in AP activity (2.0 times; р < 0.001) was observed in the periodontal homogenate of rats with LPS‑induced periodontitis. In the case of LPS‑induced periodontitis combined with chronic thiolactone HHcy, more pronounced changes in the activity of phosphatases and in MI were established as compared to rats with LPS‑induced periodontitis only.
Chronic thiolactone HHcy enhances disturbances in bone metabolism in LPS‑induced periodontitis. The osteotoxic effect of HHcy is associated with the activation of osteoclastogenesis and enhanced bone resorption. However, further research is required on the subject.
本研究旨在确定脂多糖(LPS)诱导的牙周炎合并慢性硫内酯HHcy大鼠的骨代谢特征。
四十八白色,非线性,将成熟大鼠分为4组:对照组(n=12);LPS诱导的牙周炎(n=12);慢性硫内酯HHcy(n=12);牙周炎合并HHcy(n=12)。在最后一次LPS注射后的第二天或最后一次高半胱氨酸(Hcy)硫内酯给药后的第二天处死大鼠。根据血清和牙周匀浆中碱性磷酸酶(ALP)和酸性磷酸酶(AP)的活性测定骨代谢。
在LPS诱导的牙周炎大鼠的牙周匀浆中观察到ALP活性(40.1%;中示=0.001)和矿化指数(MI)(3.5倍;中示<0.001)随着AP活性的增加(中示出2.0倍;中示出<0.001)。在LPS诱导的牙周炎合并慢性硫内酯HHcy的情况下,与仅患有LPS诱导的牙周炎的大鼠相比,磷酸酶活性和MI的变化更为明显。
慢性硫内酯HHcy增强LPS诱导的牙周炎中骨代谢紊乱.HHcy的骨毒性作用与破骨细胞生成的激活和骨吸收的增强有关。然而,这个问题需要进一步的研究。