Abstract：
Porcine reproductive and respiratory syndrome virus (PRRSV) and Mycoplasma hyopneumoniae (M. hyopneumoniae, Mhp) are two of the most common pathogens involved in the porcine respiratory disease complex (PRDC) resulting in significant economic losses worldwide. Vaccination is the most effective approach to disease prevention. Since PRRSV and Mhp co-infections are very common, an efficient dual vaccine against these pathogens is required for the global swine industry. Compared with traditional vaccines, multi-epitope vaccines have several advantages, they are comparatively easy to produce and construct, are chemically stable, and do not have an infectious potential. In this study, to develop a safe and effective vaccine, B cell and T cell epitopes of PRRSV-GP5, PRRSV-M, Mhp-P46, and Mhp-P65 protein had been screened to construct a recombinant epitope protein rEP-PM that has good hydrophilicity, strong antigenicity, and high surface accessibility, and each epitope is independent and complete. After immunization in mice, rEP-PM could induce the production of high levels of antibodies, and it had good immunoreactivity with anti-rEP-PM, anti-PRRSV, and anti-Mhp antibodies. The anti-rEP-PM antibody specifically recognizes proteins from PRRSV and Mhp. Moreover, rEP-PM induced a Th1-dominant cellular immune response in mice. Our results showed that the rEP-PM protein could be a potential candidate for the development of a safe and effective multi-epitope peptide combined vaccine to control PRRSV and Mhp infections.
摘要：
猪繁殖与呼吸综合征病毒（PRRSV）和猪肺炎支原体（M.猪肺炎,Mhp）是与猪呼吸道疾病复合体（PRDC）有关的两种最常见的病原体，在全球范围内造成重大的经济损失。接种疫苗是预防疾病最有效的方法。由于PRRSV和Mhp合并感染非常普遍，全球养猪业需要针对这些病原体的有效双重疫苗。与传统疫苗相比，多表位疫苗有几个优点，它们相对容易生产和建造，化学稳定，并且没有传染性的潜力。在这项研究中,开发一种安全有效的疫苗，PRRSV-GP5、PRRSV-M的B细胞和T细胞表位,筛选Mhp-P46和Mhp-P65蛋白,构建具有良好亲水性的重组表位蛋白rEP-PM,强抗原性,和高表面可达性，并且每个表位是独立且完整的。小鼠免疫后，rEP-PM可以诱导高水平抗体的产生，它与抗rEP-PM具有良好的免疫反应性，抗PRRSV,和抗Mhp抗体.抗rEP-PM抗体特异性识别来自PRRSV和Mhp的蛋白质。此外，rEP-PM在小鼠中诱导Th1显性细胞免疫应答。我们的结果表明，rEP-PM蛋白可能是开发安全有效的多表位肽联合疫苗以控制PRRSV和Mhp感染的潜在候选者。
