Abstract：
Vasculogenic mimicry (VM) is the ability of highly aggressive cancer cells to form fluid-conducting channels that facilitate the nutrition and metastasis of cancer cells. Considering the importance of VM in the prognosis of canine mammary gland tumours, this study aimed to investigate global gene expression in two canine mammary carcinoma cell cultures associated with the capacity for VM in vitro. The cell lines were subjected to an in-vitro assay to form VM channels (3D culture). Each cell line was then used in 2D conditions as controls and we compared the global gene expression with that of the 3D cultures. A total of 1,217 differentially expressed genes (DEGs) (P <0.05, fold change >2.0 or <2.0) were observed in 3D conditions compared with 2D culture in the UNESP-CM9 cell line, of which 677 were upregulated genes and 540 were downregulated. In contrast, the UNESP-CM60 cell line had only one upregulated and two downregulated genes. Overall, we identified several genes and pathways involved in the development of VM and these molecular data will be useful for future studies aimed at identifying diagnostic and therapeutic targets for VM in canine mammary carcinoma.
摘要：
血管生成拟态(VM)是高度侵袭性癌细胞形成促进癌细胞营养和转移的流体传导通道的能力。考虑到VM在犬乳腺肿瘤预后中的重要性，本研究旨在研究两种犬乳腺癌细胞培养物中与体外VM能力相关的整体基因表达。对细胞系进行体外测定以形成VM通道(3D培养)。然后将每个细胞系在2D条件下用作对照,并且我们将整体基因表达与3D培养物的基因表达进行比较。在UNESP-CM9细胞系中，与2D培养相比，在3D条件下观察到总共1,217个差异表达基因（DEGs）（P<0.05，倍数变化>2.0或<2.0），其中677个基因上调，540个基因下调。相比之下,UNESP-CM60细胞系只有一个上调的基因和两个下调的基因.总的来说,我们确定了与VM发展相关的几个基因和通路,这些分子数据将对旨在确定犬乳腺癌VM诊断和治疗靶点的未来研究有用.
