PDF(Sci-hub)
Abstract：
BACKGROUND: Neuroinflammation is involved in the pathophysiology of various neurological disorders, in particular Alzheimer disease (AD) and Parkinson\'s disease (PD). Alterations in the blood-brain barrier may allow peripheral blood lymphocytes to enter the central nervous system; these may participate in disease pathogenesis.
OBJECTIVE: To evaluate the peripheral blood lymphocyte profiles of patients with AD and PD and their association with the disease and its progression.
METHODS: The study included 20 patients with AD, 20 with PD, and a group of healthy individuals. Ten of the patients with AD and 12 of those with PD were evaluated a second time 17 to 27 months after the start of the study. Lymphocyte subpopulations and their activation status were determined by flow cytometry. All patients underwent neurological examinations using internationally validated scales.
RESULTS: Compared to healthy individuals, patients with AD and PD showed significantly higher levels of activated lymphocytes, lymphocytes susceptible to apoptosis, central memory T cells, and regulatory T and B cells. As the diseases progressed, there was a significant decrease in activated cells (CD4+ CD38+ and CD8+ CD38+ in PD and AD, CD4+ CD69+ and CD8+ CD69+ in PD), T cells susceptible to apoptosis, and some regulatory populations (CD19+ CD5+ IL10+ in PD and AD, CD19+ CD5+ IL10+ FoxP3+, CD4+ FoxP3+ CD25+ CD45RO+ in PD). In patients with AD, disease progression was associated with lower percentages of CD4+ CD38+ cells and higher percentages of effector CD4 cells at the beginning of the study. Significant differences were observed between both diseases.
CONCLUSIONS: This study provides evidence of changes in peripheral blood lymphocyte phenotypes associated with AD and PD and their severity. Considering effective blood-brain communication, our results open new avenues of research into immunomodulation therapies to treat these diseases.
OBJECTIVE: To evaluate the peripheral blood lymphocyte profiles of patients with AD and PD and their association with the disease and its progression.
METHODS: The study included 20 patients with AD, 20 with PD, and a group of healthy individuals. Ten of the patients with AD and 12 of those with PD were evaluated a second time 17 to 27 months after the start of the study. Lymphocyte subpopulations and their activation status were determined by flow cytometry. All patients underwent neurological examinations using internationally validated scales.
RESULTS: Compared to healthy individuals, patients with AD and PD showed significantly higher levels of activated lymphocytes, lymphocytes susceptible to apoptosis, central memory T cells, and regulatory T and B cells. As the diseases progressed, there was a significant decrease in activated cells (CD4+ CD38+ and CD8+ CD38+ in PD and AD, CD4+ CD69+ and CD8+ CD69+ in PD), T cells susceptible to apoptosis, and some regulatory populations (CD19+ CD5+ IL10+ in PD and AD, CD19+ CD5+ IL10+ FoxP3+, CD4+ FoxP3+ CD25+ CD45RO+ in PD). In patients with AD, disease progression was associated with lower percentages of CD4+ CD38+ cells and higher percentages of effector CD4 cells at the beginning of the study. Significant differences were observed between both diseases.
CONCLUSIONS: This study provides evidence of changes in peripheral blood lymphocyte phenotypes associated with AD and PD and their severity. Considering effective blood-brain communication, our results open new avenues of research into immunomodulation therapies to treat these diseases.
摘要：
背景：神经炎症与各种神经系统疾病的病理生理学有关，特别是阿尔茨海默病(AD)和帕金森病(PD)。血脑屏障的改变可能使外周血淋巴细胞进入中枢神经系统；这些可能参与疾病的发病机理。
目的：评估AD和PD患者外周血淋巴细胞谱及其与疾病和进展的关系。
方法：该研究包括20例AD患者，20和PD，和一群健康的人。研究开始后17至27个月，对10名AD患者和12名PD患者进行了第二次评估。通过流式细胞术确定淋巴细胞亚群及其活化状态。所有患者均使用国际认可的量表进行神经系统检查。
结果：与健康个体相比，患有AD和PD的患者显示出明显更高水平的活化淋巴细胞，容易凋亡的淋巴细胞，中枢记忆T细胞,以及调节性T和B细胞。随着疾病的发展，活化细胞显著减少(CD4+CD38+和CD8+CD38+在PD和AD,PD中的CD4+CD69+和CD8+CD69+），T细胞易凋亡，和一些调节性群体(PD和AD中的CD19+CD5+IL10+，CD19+CD5+IL10+FoxP3+,PD中的CD4+FoxP3+CD25+CD45RO)。在AD患者中，在研究开始时,疾病进展与CD4+CD38+细胞百分比较低和效应CD4细胞百分比较高相关.在两种疾病之间观察到显著差异。
结论：本研究提供了与AD和PD及其严重程度相关的外周血淋巴细胞表型变化的证据。考虑到有效的血脑交流，我们的研究结果为研究这些疾病的免疫调节疗法开辟了新的途径.
目的：评估AD和PD患者外周血淋巴细胞谱及其与疾病和进展的关系。
方法：该研究包括20例AD患者，20和PD，和一群健康的人。研究开始后17至27个月，对10名AD患者和12名PD患者进行了第二次评估。通过流式细胞术确定淋巴细胞亚群及其活化状态。所有患者均使用国际认可的量表进行神经系统检查。
结果：与健康个体相比，患有AD和PD的患者显示出明显更高水平的活化淋巴细胞，容易凋亡的淋巴细胞，中枢记忆T细胞,以及调节性T和B细胞。随着疾病的发展，活化细胞显著减少(CD4+CD38+和CD8+CD38+在PD和AD,PD中的CD4+CD69+和CD8+CD69+），T细胞易凋亡，和一些调节性群体(PD和AD中的CD19+CD5+IL10+，CD19+CD5+IL10+FoxP3+,PD中的CD4+FoxP3+CD25+CD45RO)。在AD患者中，在研究开始时,疾病进展与CD4+CD38+细胞百分比较低和效应CD4细胞百分比较高相关.在两种疾病之间观察到显著差异。
结论：本研究提供了与AD和PD及其严重程度相关的外周血淋巴细胞表型变化的证据。考虑到有效的血脑交流，我们的研究结果为研究这些疾病的免疫调节疗法开辟了新的途径.
