Abstract：
Periodontitis (P) is a highly prevalent inflammatory disease of the oral cavity. The objective of the study was to evaluate the stages of pro-inflammatory cytokine IL-1β in initial, moderate and severe periodontitis. One hundred and twenty two patients were included in the study. Periodontitis subjects had at least 20 natural teeth and ≥8 sites with pocket depths of >4 mm and clinical attachment loss (CAL). A questionnaire was used with respect to the socio demographic parameters which included age, gender, ethnicity, education, marital, residence and occupation. To categorize the severity of the disease, teeth were assessed for, Plaque index (PI), Bleeding on probing (BOP), CAL, missing tooth, tooth mobility and bone loss. Unstimulated whole saliva (UWS) was collected and Interleukin-1β (IL-1β) cytokine levels were analyzed using enzyme linked immunosorbent assay with microplate reader at 450 nm. Clinical parameters and salivary cytokine concentrations were assessed using one-way analysis of variance, whereas a correlation of cases with gender and severity of periodontitis was evaluated using chi-square test. Fifty-nine patients were healthy controls and 63 were periodontitis patients Thirty two percent (n = 20) had initial periodontitis, 40% (n = 25) suffered from moderate and 29% (n = 18) had severe periodontitis. Periodontitis subgroups were significantly different with regards to age and gender (p < 0.001). The mean PPD and CAL among the periodontitis patients (PPD, 3.52 ± 1.25 mm; CAL, 4.04 ± 1.64 mm) were significantly compromised (p < 0.05) compared to healthy controls (PPD, 1.52 ± 0.73 mm; CAL, 0.08 ± 0.28 mm). Increased levels of IL-1β were associated with high CAL and PPD findings. UWS IL-1β levels were higher in periodontitis patients compared to healthy individuals. In addition, cases of severe periodontitis showed significantly higher UWS IL-1β levels compared to initial and moderate periodontitis patients. Comparative levels of salivary IL-1β can be potentially used as a diagnostic tool for periodontitis identification and disease progression along with clinical parameters.
摘要：
牙周炎（P）是一种高度流行的口腔炎症性疾病。该研究的目的是评估促炎细胞因子IL-1β在初始阶段，中度和重度牙周炎。研究中包括了122名患者。牙周炎受试者有至少20颗天然牙齿和≥8个部位，口袋深度>4mm，临床附着丧失（CAL）。针对包括年龄在内的社会人口统计学参数使用了问卷，性别,种族,教育,婚姻,居住和职业。为了对疾病的严重程度进行分类，对牙齿进行了评估，斑块指数(PI),探测时放气(防喷器),CAL,缺牙,牙齿活动性和骨质流失。收集未刺激的全唾液（UWS），并使用酶联免疫吸附测定法在450nm处使用酶标仪分析白细胞介素-1β（IL-1β）细胞因子水平。使用单向方差分析评估临床参数和唾液细胞因子浓度，而使用卡方检验评估病例与性别和牙周炎严重程度的相关性。59名患者是健康对照，63名是牙周炎患者。32%(n=20)患有初始牙周炎，40％（n=25）患有中度牙周炎，29％（n=18）患有重度牙周炎。牙周炎亚组在年龄和性别方面有显著差异(p<0.001)。牙周炎患者的平均PPD和CAL(PPD，3.52±1.25mm;CAL,4.04±1.64mm）与健康对照组（PPD，1.52±0.73mm;CAL,0.08±0.28mm)。IL-1β水平升高与高CAL和PPD结果相关。与健康个体相比，牙周炎患者的UWSIL-1β水平更高。此外,与初始和中度牙周炎患者相比，重度牙周炎患者的UWSIL-1β水平显着升高。唾液IL-1β的比较水平可以潜在地用作牙周炎鉴定和疾病进展以及临床参数的诊断工具。
