UNASSIGNED: Initially, quantitative real-time polymerase chain reaction (qPCR) is employed to determine the expression of miR-551b-5p in differentiated human umbilical vein endothelial cells (HUVECs). Further, the effects of aberrantly expressed miR-551b-5p in HUVECs Transwell assay. The expressions of proteins associated with severe acute pancreatitis capillary leakage syndrome are determined by Western blot, FITC-phalloidin, and immunofluorescence stainings. Finally, the correlative factor and the target genes of miR-551b-5p, as well as their contributions, are assessed.
UNASSIGNED: We observed that overexpression of miR-551b-5p distinctly promoted the expression of EGFR, AKT3, and AQP5, while it suppressed the expression of JAM3, AQP1, and occludin. Functionally, the cytoskeleton of the miR-551b-5p overexpression was relatively loose with apparent vacuoles, and overexpression of miR-551b-5p increased the permeability of HUVECs.
UNASSIGNED: miR-551b-5p overexpression promoted changes in vascular endothelial permeability via upregulation of the EGFR/AKT3 pathway and downregulation of occludin and JAM3.
未经批准:最初,定量实时聚合酶链反应(qPCR)用于确定分化的人脐静脉内皮细胞(HUVEC)中miR-551b-5p的表达。Further,异常表达的miR-551b-5p在HUVECsTranswell试验中的作用。Westernblot检测重症急性胰腺炎毛细血管渗漏综合征相关蛋白的表达,FITC-phalloidin,和免疫荧光染色。最后,miR-551b-5p的相关因子和靶基因,以及他们的贡献,被评估。
UASSIGNED:我们观察到miR-551b-5p的过表达明显促进了EGFR的表达,AKT3和AQP5,同时抑制JAM3,AQP1和occludin的表达。功能上,miR-551b-5p过表达的细胞骨架相对松散,有明显的空泡,miR-551b-5p的过表达增加了HUVECs的通透性。
UNASSIGNED:miR-551b-5p过表达通过EGFR/AKT3通路的上调和occludin和JAM3的下调促进血管内皮通透性的变化。