Abstract：
Interactions of surface water and groundwater (SW-GW) in hyporheic zones produce biogeochemical hotspots. However, response patterns of hyporheic groundwater to external influences remain unclear. In this study, three datasets (hydrochemistry, antibiotics, and microbiome) were collected over a hydrological year to explore the influence of a 12-year managed aquifer recharge (MAR) project. We observed that the long-term MAR practice elevated nutrient and antibiotic levels while reduced redox potential in hyporheic groundwater, and these impacts depended on decreasing SW-GW interaction intensity with aquifer depth. In contrast, the long-term MAR practice increased community dissimilarity of 30-m groundwater but had little impact on 50-m or 80-m groundwater. Moreover, hyporheic community assembly was dominated by dispersal limitation, and thereby co-varied hydrochemistry and antibiotics only attributed to small community variability. The long-term MAR practice decreased species-interaction intensity and changed the abundance of metabolic functions in hyporheic groundwater. Furthermore, predicted community functions involving carbon, nitrogen, sulfur, and manganese cycles for 30-m groundwater showed higher abundances than those for 50- and 80-m groundwater. Collectively, we showed that hyporheic groundwater was sensitive to the SW-GW interaction and human activities, with the interactions of hydrochemistry, contaminants, and microbiome linking to hyporheic groundwater quality and ecosystem functioning.
摘要：
高压带地表水和地下水（SW-GW）的相互作用产生了生物地球化学热点。然而,低渗地下水对外部影响的响应模式尚不清楚。在这项研究中,三个数据集(水化学，抗生素,和微生物组）在一个水文年度内收集，以探索12年管理的含水层补给（MAR）项目的影响。我们观察到，长期的MAR实践提高了营养和抗生素水平，同时降低了高压地下水中的氧化还原电位，这些影响取决于SW-GW相互作用强度随含水层深度的降低。相比之下,长期的MAR实践增加了30米地下水的群落差异，但对50米或80米地下水影响不大。此外，充血的社区集会以分散限制为主导，从而共同变化的水化学和抗生素仅归因于小的社区变异性。长期的MAR实践降低了物种相互作用的强度，并改变了高压地下水中代谢功能的丰度。此外，预测的涉及碳的群落功能，氮，硫磺,30米地下水的锰循环显示出比50米和80米地下水更高的丰度。总的来说，我们表明，低流地下水对SW-GW相互作用和人类活动敏感，与水化学的相互作用，污染物,和微生物组与低流变地下水质量和生态系统功能相关。
