PDF(Pubmed)
Abstract：
Blueberry (Vaccinium corymbosum) is reputed as a rich source of health-promoting phytonutrients, which contributes to its burgeoning consumer demand and production. However, blueberries are much smaller and have lower yields than most domesticated berries, and the inherent regulatory mechanisms remain elusive. In this study, the cytological and physiological changes, as well as comparative transcriptomic analysis throughout flower and fruit development in the southern highbush blueberry cultivar \'O\'Neal\' were performed. \'O\'Neal\' hypanthium and fruit exhibited a distinctive cell proliferation pattern, and auxin accumulation was unusual throughout development, while abscisic acid (ABA) levels rapidly increased in association with anthocyanin accumulation, total phenolic reduction and fruit maturation. Transcriptomic data showed that many differentially expressed genes (DEGs) were specifically expressed at each flower bud and fruit developmental stage. Further weighted gene co-expression network analysis (WGCNA) revealed numerous DEGs that correlated with the cell numbers of outer mesocarp and columella, showed two distinctive expression patterns. Most of the DEGs involved in auxin biosynthesis, transportation and signal transduction were upregulated, and this upregulation was accompanied by cell expansion, and flower bud and fruit development. However, individual members of VcSAUR50 and VcIAA9 families might be insensitive to auxin, suggesting that these genes play a distinctive role in the growth and development of blueberry fruits. These results will support future research to better understand the flower and fruit development of southern highbush blueberry.
摘要：
蓝莓（Vacciniumcorymbosum）被誉为促进健康的植物营养素的丰富来源，这有助于其迅速增长的消费者需求和生产。然而,蓝莓比大多数驯化浆果小得多，产量也低，固有的监管机制仍然难以捉摸。在这项研究中,细胞学和生理变化，以及在南部高灌木蓝莓品种\'O\'Neal\'的整个花和果实发育过程中进行了比较转录组学分析。'O\'Neal\'托杯和果实表现出独特的细胞增殖模式，生长素的积累在整个发展过程中是不寻常的，而脱落酸（ABA）水平迅速增加与花青素积累有关，总酚减少和果实成熟。转录组数据表明，许多差异表达基因（DEGs）在每个花蕾和果实发育阶段都有特异性表达。进一步的加权基因共表达网络分析（WGCNA）揭示了许多与外中皮和小柱细胞数量相关的DEG，表现出两种独特的表达模式。大多数参与生长素生物合成的DEGs，运输和信号转导上调，这种上调伴随着细胞扩增，花蕾和果实发育。然而,VcSAUR50和VcIAA9家族的个别成员可能对生长素不敏感，表明这些基因在蓝莓果实的生长发育中起着独特的作用。这些结果将支持未来的研究，以更好地了解南部高灌木蓝莓的花果发育。
