Abstract：
Background: MicroRNAs (miRNAs) play an important regulatory role and serve as biomarkers in various human cancers. However, their role in the prognosis and predicting response to therapy in Indian lung cancer patients is not fully explored. Methods: We collected surgically resected tumors and paired adjacent normal lung tissues from 29 early-stage and tissue biopsies from 103 locally advanced and metastatic lung cancer patients in this prospective study. We quantified the expression levels of miR-375-3p, miR-197-3p, and miR-15a-5p using TaqMan Advanced miRNA Assays. We correlated miRNAs expression with response to therapy and survival outcomes. Results: The median age of lung cancer patients was 60 years. We found significant overexpression of miR-375-3p and miR-197-3p in the tumors compared to paired normal lung tissues. Higher expression of miR-375-3p was observed more frequently in responders compared to nonresponders. The expression of miR-375-3p and miR-197-3p was able to differentiate patients of lung adenocarcinoma from lung squamous cell carcinoma. We did not find any correlation between miRNAs expression and survival outcomes. Conclusion: Overexpression of miR-375-3p and miR-197-3p might contribute to lung carcinogenesis. The expression of miR-375-3p may assist in predicting therapeutic response. More prospective studies are warranted to evaluate the potential of miR-375-3p as a predictive biomarker of response to therapy.
摘要：
背景：MicroRNAs（miRNAs）在各种人类癌症中起着重要的调节作用，并作为生物标志物。然而,它们在印度肺癌患者预后和预测治疗反应中的作用尚未完全探讨.方法：在这项前瞻性研究中，我们从29例早期和103例局部晚期和转移性肺癌患者的组织活检中收集了手术切除的肿瘤和配对的邻近正常肺组织。我们量化了miR-375-3p的表达水平，miR-197-3p,和miR-15a-5p使用TaqMan高级miRNA检测。我们将miRNA表达与对治疗和生存结果的反应相关联。结果：肺癌患者的中位年龄为60岁。我们发现与配对的正常肺组织相比，肿瘤中miR-375-3p和miR-197-3p显著过表达。与无应答者相比,在应答者中更频繁地观察到miR-375-3p的更高表达。miR-375-3p和miR-197-3p的表达能够区分肺腺癌和肺鳞癌患者。我们没有发现miRNA表达和生存结果之间的任何相关性。结论:miR-375-3p和miR-197-3p的过表达可能与肺癌的发生有关。miR-375-3p的表达可能有助于预测治疗反应。有必要进行更多的前瞻性研究来评估miR-375-3p作为治疗反应的预测生物标志物的潜力。
