PDF(Pubmed)
Abstract：
The relationship between oral squamous cell carcinoma (OSCC) development and the microbiome has attracted increasing attention. The depth of invasion (DOI) is an important indicator of tumor progression, staging and prognosis, and the change in the oral microbiome based on the DOI is unclear. This report describes the use of metagenomic analyses to investigate the relationship between the oral microbiome and the DOI. Forty patients in different DOI categories were recruited; 10 healthy people served as the control group. Swab samples collected from the participants were subjected to metagenomic analyses, and the oral microbial communities and their functions were investigated. The abundances of Fusobacterium nucleatum, Capnocytophaga sputigena, Porphyromonas endodontalis, and Gemella haemolysans were significantly increased in the patients compared with the controls. The abundances of some bacteria exhibited a stage-related trend. The abundances of P. endodontalis, Gemella morbillorum and G. haemolysans increased with increasing DOI. In contrast, the abundances of Prevotella melaninogenica, Haemophilus parainfluenzae and Neisseria flavescens decreased with increasing DOI. Based on receiver operating characteristic (ROC) curve analysis, eight species were found to have predictive value: Rothia mucilaginosa, P. melaninogenica, H. parainfluenzae, and N. flavescens in the healthy control group and P. endodontalis, G. morbillorum, G. haemolysans and Fusobacterium periodonticum in the high DOI group. In the functional analysis, several metabolic pathways were decreased, whereas flagellar assembly and bacterial chemotaxis showed an increasing trend as the disease progressed. Biofilm formation, flagella, lipopolysaccharide (LPS) and other virulence factors exhibited staging-related changes. These pathogenic pathways and factors had a clear correlation with specific pathogens. In particular, when OSCC progressed to the late stage, microbial diversity and functional potential changed greatly.
摘要：
口腔鳞状细胞癌（OSCC）的发展与微生物组之间的关系越来越受到关注。浸润深度(DOI)是肿瘤进展的重要指标,分期和预后，基于DOI的口腔微生物组变化尚不清楚。本报告描述了使用宏基因组分析来研究口腔微生物组和DOI之间的关系。招募了40名不同DOI类别的患者；10名健康人作为对照组。从参与者那里收集的拭子样本进行了宏基因组分析，并对口腔微生物群落及其功能进行了研究。具核梭杆菌的丰度，Capnocytophagasputigena,根管卟啉单胞菌,与对照组相比，患者的血溶菌和Gemella显着增加。一些细菌的丰度表现出与阶段相关的趋势。牙髓多糖的丰度，morbillorum和G.huolysans随DOI的增加而增加。相比之下,黑色素普氏菌的丰度，副流感嗜血杆菌和黄质奈瑟菌随DOI的增加而减少。基于接收机工作特性(ROC)曲线分析,八种被发现具有预测价值：红景天，黑色素原虫，H.副流感，健康对照组和牙髓多糖，G.Morbillorum,高DOI组中的G.血溶菌和牙周梭杆菌。在功能分析中，几种代谢途径减少，而鞭毛组装和细菌趋化性显示出随着疾病进展而增加的趋势。生物膜形成,鞭毛,脂多糖（LPS）和其他毒力因子表现出分期相关的变化。这些致病途径和因素与特定病原体有明显的相关性。特别是,当OSCC进展到后期时，微生物多样性和功能潜力发生了很大变化。
