Abstract：
Highly specific and sensitive diagnostic methods are vital for the effective control and treatment of toxoplasmosis. Routine diagnosis is primarily serological because T. gondii infections stimulate persistently high IgG antibody responses. The sensitivity and specificity of methods are crucial factors for the proper diagnosis of toxoplasmosis, primarily dependent on the antigens used in different assays. In the present study, we compared the serodiagnostic performances of three recombinant dense granule antigens, namely, the GRA6, GRA7, and GRA14, to detect IgG antibodies against T. gondii in human sera from the Philippines. Moreover, we evaluated the IgG1, IgG2, IgG3, and IgG4 responses against the different recombinant antigens, which has not been performed previously. Our results revealed that the TgGRA7 has consistently displayed superior diagnostic capability, while TgGRA6 can be a satisfactory alternative antigen among the GRA proteins. Furthermore, IgG1 is the predominant subclass stimulated by the different recombinant antigens. This study\'s results provide options to researchers and manufacturers to choose recombinant antigens suitable for their purpose.
摘要：
高度特异性和敏感性的诊断方法对于有效控制和治疗弓形虫病至关重要。常规诊断主要是血清学诊断,因为弓形虫感染刺激持续的高IgG抗体应答。方法的敏感性和特异性是正确诊断弓形虫病的关键因素。主要依赖于不同分析中使用的抗原。在本研究中,我们比较了三种重组致密颗粒抗原的血清诊断性能，即,GRA6，GRA7和GRA14，用于检测来自菲律宾的人血清中针对弓形虫的IgG抗体。此外，我们评估了IgG1，IgG2，IgG3和IgG4对不同重组抗原的反应，以前没有执行过。我们的结果表明，TgGRA7始终显示出卓越的诊断能力，而TgGRA6可以是GRA蛋白中令人满意的替代抗原。此外，IgG1是由不同重组抗原刺激的主要亚类。这项研究的结果为研究人员和制造商选择适合其目的的重组抗原提供了选择。
