Abstract：
Porcine circovirus 3 (PCV-3) has been associated with several pig diseases. Despite the pathogenicity of this virus has not been completely clarified, reproductive disorders are consistently associated with its infection. The aim of the present work was to analyze the presence of PCV-3 DNA in tissues from pig fetuses from different gestational timepoints. The fetuses were obtained either from farms with no reproductive problems (NRP, n = 249; all of them from the last third of gestation) or from a slaughterhouse (S, n = 51; 49 of the second-third of gestation and 2 from the third one). Tissues collected included brain, heart, lung, kidney, and/or spleen. Overall, the frequency of detection of PCV-3 was significantly higher in fetuses from the last third of the gestation (69/251, 27.5%) when compared to those from the second-third (5/49, 10.2%), although the viral loads were not significantly different. Moreover, the frequency of detection in NRP fetuses (69/249, 27.7%) was significantly higher than in S ones (5/51, 9.8%). Furthermore, PCV-3 DNA was detected in all tissue types analyzed. In conclusion, the present study demonstrates a higher frequency of PCV-3 DNA detection in fetuses from late periods of the gestation and highlights wide organ distributions of the virus in pig fetuses.
摘要：
猪圆环病毒3（PCV-3）与几种猪疾病有关。尽管这种病毒的致病性尚未完全阐明，生殖疾病始终与其感染有关。本工作的目的是分析来自不同妊娠时间点的猪胎儿的组织中PCV-3DNA的存在。胎儿是从没有生殖问题的农场获得的(NRP，n=249；所有这些都来自妊娠的最后三分之一）或屠宰场（S，n=51；妊娠第二至三分之一的49和第三妊娠的2）。收集的组织包括大脑，心,肺，肾,和/或脾脏。总的来说,与妊娠后三分之一的胎儿（69/251，27.5%）相比，妊娠后三分之一的胎儿（5/49，10.2%）检测到PCV-3的频率明显更高，尽管病毒载量没有显著差异.此外，NRP胎儿的检测频率（69/249，27.7％）显着高于S胎儿（5/51，9.8％）。此外，在所分析的所有组织类型中检测到PCV-3DNA。总之,本研究表明,在妊娠后期的胎儿中检测PCV-3DNA的频率更高,并强调了猪胎儿中病毒的广泛器官分布.
