Abstract：
Loss of the polybromo-1 (PBRM1) protein has been expected as a possible biomarker for clear cell renal cell carcinoma (ccRCC). There is little knowledge about how PBRM1 immunohistochemical expression correlates with the histomorphological features of ccRCC and the endothelial expression of tumor vasculature. The present study evaluates the association of architectural patterns with the PBRM1 expression of cancer cells using a cohort of 425 patients with nonmetastatic ccRCC. Furthermore, we separately assessed the PBRM1 expression of the endothelial cells and evaluated the correlation between the expression of cancer cells and endothelial cells. PBRM1 loss in cancer cells was observed in 148 (34.8%) patients. In the correlation analysis between architectural patterns and PBRM1 expression, macrocyst/microcystic, tubular/acinar, and compact/small nested were positively correlated with PBRM1 expression, whereas alveolar/large nested, thick trabecular/insular, papillary/pseudopapillary, solid sheets, and sarcomatoid/rhabdoid were negatively correlated with PBRM1 expression. PBRM1 expression in vascular endothelial cells correlated with the expression of cancer cells (correlation coefficient = 0.834, p < 0.001). PBRM1 loss in both cancer and endothelial cells was associated with a lower recurrence-free survival rate (p < 0.001). Our PBRM1 expression profile indicated that PBRM1 expression in both cancer and endothelial cells may be regulated in an orchestrated manner.
摘要：
聚溴-1（PBRM1）蛋白的缺失已被预期为透明细胞肾细胞癌（ccRCC）的可能生物标志物。关于PBRM1免疫组织化学表达如何与ccRCC的组织形态学特征和肿瘤脉管系统的内皮表达相关的知识很少。本研究使用425例非转移性ccRCC患者的队列评估了结构模式与癌细胞PBRM1表达的关联。此外，我们分别评估了内皮细胞的PBRM1表达，并评估了癌细胞与内皮细胞表达之间的相关性。在148例（34.8％）患者中观察到PBRM1在癌细胞中的丢失。在建筑模式与PBRM1表达的相关性分析中，大囊肿/微囊，管状/腺泡，紧密/小嵌套与PBRM1表达呈正相关，而肺泡/大嵌套，厚的小梁/岛叶，乳头状/假乳头状，实心薄板,肉瘤样/横纹肌样均与PBRM1表达呈负相关。PBRM1在血管内皮细胞中的表达与癌细胞的表达相关(相关系数=0.834,p<0.001)。癌症和内皮细胞中PBRM1的丢失与较低的无复发生存率相关(p<0.001)。我们的PBRM1表达谱表明，PBRM1在癌细胞和内皮细胞中的表达可能以协调的方式受到调节。
