Abstract：
Allelochemicals are considered an environment-friendly and promising alternative for weed management, although much effort is still needed for understanding their mode of action and then promoting their use in plant allelopathy management practices. Here, we report that Inuloxin A (InA), an allelochemical isolated from Dittrichia viscosa, inhibited root elongation and growth of seedlings of Lycopersicon esculentum and Lepidium sativum at the highest concentrations tested. InA-induced antioxidant responses in the seedlings were investigated by analysing the contents of glutathione (GSH) and ascorbate (ASC), and their oxidized forms, dehydroascorbate (DHA), and glutathione disulphide (GSSG), as well as the redox state of thiol-containing proteins. An increase in ASC, DHA, and GSH levels at high concentrations of InA, after 3 and 6 days, were observed. Moreover, the ASC/DHA + ASC and GSH/GSSG + GSH ratios showed a shift towards the oxidized form. Our study provides the first insight into how the cell redox system responds and adapts to InA phytotoxicity, providing a framework for further molecular studies.
摘要：
化感化学品被认为是一种环保且有前途的杂草管理替代品，尽管仍需要付出很多努力来了解它们的作用方式，然后促进它们在植物化感作用管理实践中的使用。这里,我们报道了InuloxinA(InA)，一种从粘胶滴虫中分离出的化感化学物质，在测试的最高浓度下，抑制了Lycopersiconesculentum和Lepidium的根伸长和幼苗的生长。通过分析谷胱甘肽（GSH）和抗坏血酸（ASC）的含量，研究了InA诱导的幼苗抗氧化反应。以及它们的氧化形式，脱氢抗坏血酸（DHA），和谷胱甘肽二硫化物（GSSG），以及含巯基蛋白质的氧化还原状态。ASC的增加，DHA,和高浓度InA时的GSH水平，3和6天后，被观察到。此外，ASC/DHA+ASC和GSH/GSSG+GSH比率显示向氧化形式的转变。我们的研究提供了细胞氧化还原系统如何响应和适应InA植物毒性的第一个见解，为进一步的分子研究提供框架。
