Abstract：
One of the most valuable practices for analyzing not-so-analytical-friendly analytes in complex, heterogenous matrices is derivatization. Availability of numerous derivatizing reagents (DRs) makes the modification of analyte more exploitable in terms of an analytical perspective. A wide array of derivatization techniques like pre or post-column, in-situ, enzymatic, ultrasound-assisted, microwave-assisted, photochemical derivatization has added much-needed methodological strength in analyzing intricate analytical matrices (food, water, and soil). In recent years, analytical chemistry has achieved greater heights through the development of new sensitive methods with simple conventional instruments like High-Performance Liquid Chromatography (HPLC) devoid of Mass detectors. The prompt availability of these straightforward instruments also makes it a favorable option for routine analysis in food, environmental, bioanalytical chemistry. Analyzing food, environmental or bioanalytical specimen has some of the most problematic aspects, like the low concentration of the analytes accompanied by not too suitable analytical properties. Even though conventional HPLC lacks the required sensitivity but merger with derivatization can lead to a remarkable increase in sensitivity. In recent years there has been a lot of application of diverse derivatizations to increase the sensitivity and selectivity of the analyte for available instruments, resulting in notable findings. Therefore, this review describes the application of derivatization principles in the analysis of analytes in food and additional matrices using conventional HPLC instruments such as HPLC-UV, HPLC-DAD, and HPLC-FD. In this article, we will briefly review the different modes and multiple types of derivatizing reagents with their mechanisms and importance for encouraging the use of established HPLC instruments.
摘要：
分析复杂分析中不那么友好的分析物的最有价值的实践之一，异质矩阵是衍生化的。许多衍生试剂（DR）的可用性使分析物的修饰在分析角度上更可利用。一系列广泛的衍生技术，如柱前或柱后，就地,酶，超声辅助,微波辅助，光化学衍生化在分析复杂的分析基质(食品，水,和土壤）。近年来,通过使用简单的常规仪器（如高效液相色谱（HPLC），没有质谱检测器）开发新的灵敏方法，分析化学已经达到了更高的高度。这些简单的仪器的迅速可用性也使其成为食品常规分析的有利选择，环境,生物分析化学.分析食物,环境或生物分析标本有一些最有问题的方面，如低浓度的分析物伴随着不太合适的分析特性。即使常规HPLC缺乏所需的灵敏度，但与衍生化合并可导致灵敏度显着提高。近年来，已经有很多不同的衍生化的应用，以提高灵敏度和选择性的分析物的现有仪器，产生了显著的发现。因此,这篇综述描述了衍生原理在使用常规HPLC仪器（如HPLC-UV）分析食品和其他基质中分析物的应用，HPLC-DAD,和HPLC-FD。在这篇文章中,我们将简要回顾不同模式和多种类型的衍生化试剂及其机制和重要性，鼓励使用已建立的HPLC仪器。
