Abstract：
Gastric cancer (GC) has a great fatality rate, meanwhile, there is still a lack of available biomarkers for prognosis. The goal of the research was to discover key and novel potential biomarkers for GC. We screened for the expression of significantly altered genes based on survival rates from two consensus molecular subtypes (CMS) of GC. Subsequently, functional enrichment analysis showed these genes involved in many cancers. And we picked 6 hub genes that could both secreted in the tumor microenvironment and expression enhanced in immune cells. Then, Kaplan Meier survival and expression detected in the tumor pathological stage were utilized to clarify the prognostic of these 6 hub genes. The results indicated that OGN, CHRDL2, C2orf40, THBS4, CHRDL1, and ANGPTL1, respectively, were significantly associated with poor OS in GC patients. And their expression increased with cancer advanced. Moreover, immune infiltration analysis displayed that those hub genes expression positively with M2 macrophage, CD8+ T Cell, most immune inhibitors, and majority immunostimulators. In summary, our results suggested that OGN, CHRDL2, C2orf40, THBS4, CHRDL1, and ANGPTL1 were all potential biomarkers for GC prognosis and might also be potential therapeutic targets for GC.
摘要：
胃癌（GC）有很大的致死率，同时,目前仍缺乏可用于预后的生物标志物.这项研究的目的是发现GC的关键和新的潜在生物标志物。我们基于来自GC的两个共有分子亚型(CMS)的存活率来筛选显著改变的基因的表达。随后，功能富集分析显示这些基因与许多癌症有关。我们选择了6个hub基因，它们既可以在肿瘤微环境中分泌，又可以在免疫细胞中增强表达。然后，在肿瘤病理阶段检测到的KaplanMeier存活和表达用于阐明这6个hub基因的预后。结果表明，OGN，分别为CHRDL2、C2orf40、THBS4、CHRDL1和ANGPTL1，与GC患者OS差显著相关。它们的表达随癌症进展而增加。此外，免疫浸润分析表明，这些hub基因与M2巨噬细胞呈阳性表达，CD8+T细胞，大多数免疫抑制剂，和大多数免疫刺激剂。总之,我们的结果表明，OGN，CHRDL2、C2orf40、THBS4、CHRDL1和ANGPTL1都是GC预后的潜在生物标志物，也可能是GC的潜在治疗靶点。
